Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-313

Background :

The NF-kappaB inhibitor, IkappaB, has classically been characterized for its ability to sequester NF-kappaB transcription factors in the cytoplasm.

Description :

Recombinant Human I-kappa-Beta-alpha (amino acids 1-317) is produced in E. coli with an aminoterminal polyhistidine tag and purified via sequential chromatography. Recombinant Human IκBα is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant IκBα is supplied as lyophilized freeze dry powder without additives.

Storage:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Biological Activity:

Recombinant human IκBα (Inhibitor of Nuclear Factor (NF)-κB, α isoform) is phosphorylatable in vitro, using either recombinant active IKKα or IKKβ, or with immunoprecipitated IKK from stimulated cells. This phosphorylation can be monitored by ELISA directed to IκBα pS32 or by Western blotting using phosphorylation site specific antibodies directed to IκBα pS32 or pS36 in conjunction with chemiluminescence detection methods.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:NFKBIA
Synonyms:IKBA, MAD3, NFKBI

Protein synonyms/aliases:

NF-kappaB inhibitor alpha (Major histocompatibility complex enhancer-binding protein MAD3)
    (I-kappa-B-alpha)
    (IkappaBalpha)
    (IKB-alpha).

Protein Family:

Belongs to the NF-kappa-B inhibitor family.

Protein Domains:

Domains:
•IPR002110   Ankyrin

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Roles of Ras and extracellular signal-regulated kinase-dependent IkappaBalpha degradation in oridonin-enhanced phagocytosis of apoptotic cells by human macrophage-like U937 cells.
Int Immunopharmacol 2006 Feb;6(2):260-268
2. In vivo and in vitro sensitization of leukemic cells to adriamycin-induced apoptosis by pentoxifylline Involvement of caspase cascades and IkappaBalpha phosphorylation.
Immunol Lett 2006 Mar 15;103(2):149-58
3. In Vitro SCF(beta-Trcp1)-Mediated IkappaBalpha Ubiquitination Assay for High-Throughput Screen.
Methods Enzymol 2005;399:729-40
4. Minocycline exerts inhibitory effects on multiple mitogen-activated protein kinases and IkappaBalpha degradation in a stimulus-specific manner in microgl ia.
J Neurochem 2006 Jan;96(2):314-23
5. Inhibition of nuclear translocation of nuclear factor-kappaB despite lack of functional IkappaBalpha protein overcomes multiple defects in apoptosis signaling in human B-cell malignancies.
Clin Cancer Res 2005 Nov 15;11(22):8186-94
6. The synthetic epoxyquinoids jesterone dimer and epoxyquinone A monomer induce apoptosis and inhibit REL (human c-Rel) DNA binding in an IkappaBalpha-deficient diffuse large B-cell lymphoma cell line.
Cancer Lett 2005 Nov 12;

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中華民國95年06月06日更新