Source:
>
E.Coli
Catalog No.
CHM-327
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Solubility: It is recommended to reconstitute the lyophilized hIL-8 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Interleukin-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rhIL-8 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Lys-Glu-Leu.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rHuIL-8 is fully biologically active when compared to standard. Specific Activity of IL-8 in chemotaxis of donor PBL neutrophils, threshold concentration corresponding to 25-150 ng/ml.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of IL-8.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Eotaxin as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:IL8
Interleukin-8 precursor (IL-8)
(CXCL8)
(Monocyte-derived neutrophilchemotactic factor)
(MDNCF)
(T-cell chemotactic factor)
(Neutrophil-activating protein 1)
(NAP-1)
(Protein 3-10C)
(Granulocyte chemotacticprotein 1)
(GCP-1)
(Monocyte derived neutrophil-activating peptide)
(MONAP)
(Emoctakin)
MDNCF-a (IL8/NAP1 form I)
(GCP/IL-8protein IV)
Interleukin-8 (IL-8(1-77))
(MDNCF-b)
(IL8/NAP1 form II)
(GCP/IL-8 protein II)
((Ala-IL-8)77)
IL-8(5-77)
IL-8(6-77)
(Lymphocyte-derived neutrophil-activating factor)
(LYNAP)
(Neutrophil-activating factor)
(NAF)
(MDNCF-c)
(IL8/NAP1 form III)
(GCP/IL-8protein I)
((Ser-IL-8)72)
IL-8(7-77)
(IL8/NAP1 form IV)
(GCP/IL-8protein V)
IL-8(8-77)
(IL8/NAP1 form V)
(GCP/IL-8 protein VI)
IL-8(9-77)
(IL8/NAP1 form VI)
(GCP/IL-8 protein III) .
Belongs to the intercrine alpha (chemokine CxC)
Domains:
•IPR001811 Small chemokine, interleukin-8-like
