Source:
E.Coli
Catalog No.
CYT-203
IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-ra.
The regulated expression of IL1ra in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1ra is markedly enhanced by IL-1 , TNF-alpha , or PDGF.
Description : Recombinant Human IL-1ra produced in E.Coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153amino acids and having a molecular mass of 17000 Dalton Recombinant IL-1ra is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized protein with no additives.
Solubility: It is recommended to reconstitute the lyophilized IL-1ra in sterile 18Mω-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized IL-1ra although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-1ra should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's Interleukin-1ra is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of IL-1ra.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-1ra as a Reference Standard.
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:IL1RN
Synonyms:IL1F3, IL1RA
Interleukin-1 receptor antagonist protein precursor (IL-1ra)
(IRAP)
(IL1 inhibitor)
(IL-1RN)
(ICIL-1RA).
Belongs to the IL-1 family.
Domains:
•IPR000975 Interleukin-1
•Recombinant Human Interleukin-18
•Recombinant Mouse Interleukin-18
•Recombinant Rat Interleukin-18
•Recombinant Human Interleukin-1 alpha
•Recombinant Human Interleukin-1 beta
•Recombinant Murine Interleukin-1 beta
