Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CYT-300

Background:

Insulin Like Growth Factor Binding Protein-3 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. IGFBP-3 is the major IGF binding protein present in serum of humans and animals. It is also present in the alpha granules of platelets. IGFBP-3 shows a similar affinity for IGF-1 and IGF-2 . IGF-BP-3 inhibits follicle stimulating hormone . Markedly decreased levels of IGF-BP-3 are observed in patients with Growth hormone deficiencies, while markedly elevated levels are observed in patients with high levels of Growth hormone (acromegaly). In murine fibroblasts the synthesis of IGFBP-3 is stimulated by mitogenic growth factors such as Bombesin , Vasopressin , PDGF , and EGF . In human skin fibroblasts the synthesis of IGFBP-3 is stimulated by TGF-beta .

Description :

Recombinant Human IGFBP-3 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 264 amino acids and having a molecular mass of 28806 Dalton. IGFBP-3 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

IGFBP-3 was lyophilized with 20mM Tris HCL .

Solubility:

It is recommended to reconstitute the lyophilized IGFBP-3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized IGFBP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IGFBP-3 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Purity:

The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Ala-Ser-Ser-Gly.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's IGFBP-3 is fully biologically active when compared to standards. The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is less then 0.2 ug/ml in the presence of 15 ng/ml of Human IGF-II.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of IGFBP-3 .

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of IGFBP-3 as a Reference Standard.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:IGFBP3
Synonyms:IBP3

Protein synonyms/aliases:

Insulin-like growth factor binding protein 3 precursor (IGFBP-3)
    (IBP-3)
    (IGF-binding protein 3).

Protein Family:

Protein Domains:

Domains:
•IPR000867   Insulin-like growth factor-binding protein, IGFBP
•IPR000716   Thyroglobulin type-1

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Polymorphisms of genes coding for insulin-like growth factor 1 and its major binding proteins, circulating levels of IGF-I and IGFBP-3 and breast cancer risk: results from the EPIC study.
Br J Cancer 2006 Jan 30;94(2):299-307
2. A prospective study of serum IGF-I and IGFBP-3 in 942 healthy infants: Associations with birth weight, gender, growth velocity and breastfeeding.
J Clin Endocrinol Metab 2006 Jan 4;
3. Dihydrotestosterone and Testosterone, but not DHEA or Estradiol, Differentially Modulate IGF-I, IGFBP - 2 and IGFBP-3 Gene and Protein Expression in Primary Cultures of Human Prostatic Stromal Cells.
Am J Physiol Endocrinol Metab 2005 Dec 20;
4. Relationship between insulin-like growth factors (IGF-I and IGF-II), IGF-binding proteins (IGFBP-3, IGFBP-2), leptin and anthropometric parameters (height, body mass index) during antileukaemic treatment in children.
Rocz Akad Med Bialymst 2005;50:208-11
5. Serum zinc, insulin-like growth factor-I and insulin-like growth factor binding protein-3 levels in children with type 1 diabetes mellitus.
J Pediatr Endocrinol Metab 2005 Oct;18(10):1007-11
6. IGF-1, IGFBP-3, and nutritional factors in young black and white men: the CARDIA Male Hormone Study.
Nutr Cancer 2005;53(1):57-64

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中華民國95年06月06日更新