Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-383

Background :

ICAM-1 (CD54) is a single chain membrane glycoprotein expressed on the surface of a variety of non-haematopoietic and haematopoietic cell types and has roles in signal transduction, cell signalling and lymphocyte adhesion. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory conditions. Intercellular adhesion molecule 1 (ICAM-1) is the cellular receptor for the major group of human rhinovirus serotypes, including human rhinovirus 14 (HRV14) and HRV16.

Description :

Recombinant Human ICAM1 is expressed in E. coli containing 455 amino acids 28-483 fused to an amino terminal hexahistidine tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Intercellular Adhesion Molecule-1 is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Stability:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Applications:

Recombinant ICAM-I can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
The biological activity of this product has not yet been tested.

Purity:

Greater than 95% (SDS-PAGE). Single band on Western Blot.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:ICAM1

Protein synonyms/aliases:

Intercellular adhesion molecule 1 precursor (ICAM-1)
    (Major grouprhinovirus receptor)
    (CD54 antigen).

Protein Family:

Belongs to the immunoglobulin superfamily. ICAM

Protein Domains:

Domains:
•IPR003987   Intercellular adhesion molecule/vascular cell adhesion molecule-1

Related proteins:

• Recombinant Human Killer Cell Immunoglobulin-Like Receptor 2DL1
•Recombinant Human Killer Cell Immunoglobulin-Like Receptor 2DL3
• Recombinant Human Killer Cell Immunoglobulin-Like Receptor 2DS4
•Recombinant Human Carcinoembryonic Antigen

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Sodium salicylate inhibits TNF-alpha-induced NF-kappaB activation, cell migration, invasion and ICAM-1 expression in human melanoma cells.
Melanoma Res 2006 Feb;16(1):11-22
2. Glucosamine Sulfate Inhibits TNF-{alpha} and IFN-{gamma}-Induced Production of ICAM-1 in Human Retinal Pigment Epithelial Cells In Vitro.
Invest Ophthalmol Vis Sci 2006 Feb;47(2):664-72
3. Difference in adhesion molecule expression (ICAM-1 and VCAM-1) in juvenile and adult dermatomyositis, polymyositis and inclusion body myositis.
Autoimmun Rev 2006 Feb;5(2):93-100
4. Selective regulation of MAP kinases and Chemokine expression after ligation of ICAM-1 on human airway epithelial cells.
Respir Res 2006 Jan 23;7(1):12
5. Lymphocyte transcellular migration occurs through recruitment of endothelial ICAM-1 to caveola- and F-actin-rich domains.
Nat Cell Biol 2006 Jan 22;
6. Nontypeable Haemophilus influenzae Adheres to Intercellular Adhesion Molecule 1 (ICAM-1) on Respiratory Epithelial Cells and Upregulates ICAM-1 Expression.
Infect Immun 2006 Feb;74(2):830-8

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中華民國95年06月06日更新