Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-312

Description :

Recombinant Human I-309 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8504 Dalton.
Human I-309 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Human I-309 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized Human I-309 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized I-309 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Human I-309 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Ser-Met-Gln.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

PProSpec's Human I-309 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human T cells at 10.0-100.0 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human HCC-1.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Human I-309 as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CCL1
Synonyms:SCYA1

Protein synonyms/aliases:

Small inducible cytokine A1 precursor (CCL1)
(T lymphocyte-secretedprotein I-309).

Protein Family:

Belongs to the intercrine beta (chemokine CC) family.

Protein Domains:

Domains:
•IPR001811 Small chemokine, interleukin-8-like

Related proteins:

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Human cord blood-derived mast cells synthesize and release I-309 in response to IgE.
Life Sci 2003 Oct 3;73(20):2571-81
2. CCR8-dependent activation of the RAS/MAPK pathway mediates anti-apoptotic activity of I-309/ CCL1 and vMIP-I.
Eur J Immunol 2003 Feb;33(2):494-501
3. Autocrine antiapoptotic stimulation of cultured adult T-cell leukemia cells by overexpression of the chemokine I-309.
Blood 2001 Aug 15;98(4):1150-9
4. Human NK cells express CC chemokine receptors 4 and 8 and respond to thymus and activation-regulated chemokine, macrophage-derived chemokine, and I-309.
J Immunol 2000 Apr 15;164(8):4048-54
5. The chemokine receptor CCR8 mediates human endothelial cell chemotaxis induced by I-309 and Kaposi sarcoma herpesvirus-encoded vMIP-I and by lipoprotein(a)-stimulated endothelial cell conditioned medium.
Blood 2001 Jan 1;97(1):39-45
6. I-309 binds to and activates endothelial cell functions and acts as an angiogenic molecule in vivo.
Blood 2000 Dec 15;96(13):4039-45

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中華民國95年06月06日更新