Source:
E.Coli
Catalog No.
CYT-235
Human placental GH (hPGH) replaces pituitary GH during pregnancy. hPGH is correlated to serum IGF-I in normal pregnancies and in pregnancies complicated by fetoplacental disorders Description: Recombinant Placental Human GH-22K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22367 Dalton. Predicted pI=7.80.
Recombinant Human GH-22K-pl and Recombinant Human GH-22K-pit have similar biological activity mediated through human growth hormone receptors. However Recombinant GH-22K-pl is completely devoid of any lactogenic (prolactin receptor mediated) activity in homologous system consisting of human prolactin receptors, an activity which is characteristic of human pituitary 22 K and 20 K GHs. Thus, in contrast to pituitary hGHs it can be preferably used in any system in which the accompanying lactogenic activity is not wanted.
Recombinant Human GH-22K-pl is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 8-9.
Solubility: It is recommended to reconstitute the lyophilized Recombinant GH-22K-pl in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Human GH-22K-pl although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution and filter sterilization Recombinant GH-22K-pl can be stored at 40C for up to 4 weeks.
For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be
Ala-Phe-Pro-Thr-Ile. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec旧 Recombinant Human Placental Growth Hormone-22K is fully biologically active when compared to World Health Organization (WHO) reference standard.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Recombinant Placental Growth Hormone.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.18 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH-22K-pl as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:GH2
Growth hormone variant precursor (GH-V)
(Placenta-specific growthhormone)
(Growth hormone 2).
Belongs to the somatotropin/prolactin family.
Domains:
•IPR001400 Somatotropin hormone
•Recombinant Human Prolactin
•Recombinant Ovine Prolactin
•Recombinant Human Pituitary Growth Hormone-20K
•Recombinant Rat Growth Hormone
•Recombinant Ovine Growth Hormone
•Recombinant bream Growth Hormone
