Certificate of Analysis and Data Sheet

Source:
Baculovirus, SF9 insect cells

Catalog No.
PKA-312

Background :

Focal adhesion kinase (FAK) is a critical mediator of signal transduction by integrins and growth factor receptors in a variety of cells including endothelial cells (ECs).It is a nonreceptor protein tyrosine kinase involved in signaling downstream of integrins, linking bacterial detection, cell entry, and initiation of proinflammatory response through MAPKs and NF-kappaB activation.

Description :

Recombinant human FAK (amino acids 2 to 1052) is produced in Sf9 cells with an amino-terminal polyhistidine tag and purified via sequential chromatography.

Formulation:

rHuFAKis supplied at a concentration of 0.1mg/ml in 40mM Tris, pH 7.5, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.1mM EGTA, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate, and 0.03% Brij-35.

Storage:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Biological Activity:

Recombinant Human FAK contains14units/mg. One unit of FAK activity transfers 1 nmole of phosphate to poly [GluTyr] substrate per minute at 30蚓 in a reaction mixture containing 8mM ATP. Substrates for FAK include STAT, paxillin, p85 PI 3-kinase, Cas, and tensin.FAK also undergoes autophosphorylation.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:PTK2
Synonyms:FAK, FAK1

Protein synonyms/aliases:

Focal adhesion kinase 1 (EC 2.7.1.112)
    (FADK 1)
    (pp125FAK)
    (Protein-tyrosine kinase 2).

Protein Family:

Belongs to the Tyr protein kinase family. FAK

Protein Domains:

Domains:
•IPR000299   Band 4.1
•IPR000299   Band 4.1
•IPR000719   Protein kinase
•IPR005189   Focal adhesion targeting region

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Clinical significance of focal adhesion kinase in resectable pancreatic cancer.
World J Surg 2006 Feb;30(2):219-26
2. Angiopoietin 2 induces glioma cell invasion by stimulating matrix metalloprotease 2 expression through the alphavbeta1 integrin and focal adhesion kinase signaling pathway.
Cancer Res 2006 Jan 15;66(2):775-83
3. Focal adhesion kinase is critical for entry of Kaposi's sarcoma-associated herpesvirus into target cells.
J Virol 2006 Feb;80(3):1167-80
4. Role of c-Src and focal adhesion kinase in progression and metastasis of estrogen receptor-positive breast cancer.
Biochem Biophys Res Commun 2006 Mar 3;341(1):73-81
5. [The expression of tumor suppressor gene PTEN protein and focal adhesion kinase in salivary adenoid cystic carcinoma and its significance.]
Shanghai Kou Qiang Yi Xue 2005 Dec;14(6):578-81
6. Mechanisms of focal adhesion kinase regulation.
Curr Cancer Drug Targets 2005 Dec;5(8):629-43

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中華民國95年06月06日更新