Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-417

Background:

Human Fatty Epidermal Acid Binding Protein FABP also called FABP-5 is a 15 kD member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds ( bile acids or retinoids). in an internal cavity. The fatty acid binding proteins aP2 (fatty acid binding protein [FABP]-4) and mal1 (EFABP) are closely related and both are expressed in adipocytes. Absence of EFABP/mal1 resulted in increased systemic insulin sensitivity in two models of obesity and insulin resistance. Adipocytes isolated from mal1-deficient mice also exhibited enhanced insulin-stimulated glucose transport capacity. In contrast, mice expressing high levels of mal1 in adipose tissue display reduced systematic insulin activity.

Description:

Recombinant Human Epidermal Fatty Acid Binding Protein(FABP-5) is a monodimeric, non-glycosylated, polypeptide chain containing 135 amino acids and having a total molecular mass of 15200 Daltons.

Physical Appearance:

Sterile Filtered lyophilized (freeze-dried) powder.

Formulation:

Sterile filtered and lyophilized from 0.5 mg/ml in phosphate buffered saline.

Specificity:

The amino acid sequence of the recombinant human FABP5 is 100% homologous to the amino acid sequence of the human FABP-5.

Purification Method:

Two-step procedure using size exclusion chromatography before and after refolding.

Purity:

>90% (SDS-PAGE analyzed)

Reconstitution:

Add 0.2 ml of dH20 and let the lyophilized pellet dissolve completely.

Storage:

Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid freeze-thaw cycles.
Reconstituted protein can be stored at 4蚓 for a limited period of time; it does not show any change after two weeks at 4蚓.

Stability/Shelf Life:

The lyophilized protein remains stable until the expiry date when stored at -20蚓.

Applications:

Western blotting, ELISA

References:

?Maeda K, Uysal KT, Makowski L, Gorgun CZ, Atsumi G, Parker RA, Bruning J, Hertzel AV, Bernlohr DA, Hotamisligil GS. Role of the fatty acid binding protein mal1 in obesity and insulin resistance. Diabetes. 2003 Feb;52(2):300-7.
?Fisher RM, Hoffstedt J, Hotamisligil GS, Thorne A, Ryden M. Effects of obesity and weight loss on the expression of proteins involved in fatty acid metabolism in human adipose tissue. Int J Obes Relat Metab Disord. 2002 Oct;26(10):1379-85.
?Jenkins-Kruchten AE, Bennaars-Eiden A, Ross JR, Shen WJ, Kraemer FB, Bernlohr DA. Fatty acid-binding protein-hormone-sensitive lipase interaction. Fatty acid dependence on binding.J Biol Chem. 2003 Nov 28;278(48):47636-43.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:FABP5

Protein synonyms/aliases:

Fatty acid-binding protein, epidermal (E-FABP)
    (Psoriasis-associatedfatty acid-binding protein homolog)
    (PA-FABP).

Protein Family:

Belongs to the fatty-acid binding protein (FABP)

Protein Domains:

Domains:
•IPR000566   Lipocalin-related protein and Bos/Can/Equ allergen

Related proteins:

•Recombinant Human Adipocyte Fatty Acid Binding Protein (FABP-4)
•Recombinant Human Fatty Acid Binding Protein

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Decreased keratinocyte motility in skin wound on mice lacking the epidermal fatty acid binding protein gene.
Mol Cell Biochem 2006 Jan 13;:1-6
2. Epidermal fatty acid-binding protein is increased in rat lungs following in vivo treatment with keratinocyte growth factor.
Int J Biochem Cell Biol 2006 Feb;38(2):279-87
3. S100A7 (psoriasin) interacts with epidermal fatty acid binding protein and localizes in focal adhesion-like structures in cultured keratinocytes.
J Invest Dermatol 2003 Jul;121(1):132-41
4. Lack of upregulation of epidermal fatty acid binding protein in dithranol induced irritation.
Eur J Dermatol 2003 May-Jun;13(3):254-7
5. Induction of epidermal fatty acid binding protein in intravascular monocytes of renal allografts.
Transplantation 2003 Mar 15;75(5):685-8
6. Increased expression of epidermal fatty acid binding protein, cofilin, and 14-3-3-sigma (stratifin) detected by two-dimensional gel electrophoresis, mass spectrometry and microsequencing of drug-resistant human adenocarcinoma of the pancreas.
Electrophoresis 1999 Oct;20(14):2952-60

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新