Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-340

Background:

Recombinant Fatty Acid Binding Protein is a newly introduced plasma marker of acute myocardial infarction (AMI). The plasma kinetics of FABP (15kD) closely resemble those of myoglobin in that elevated plasma concentrations are found within 2 hours after AMI and return to normal generally within 18 to 24 hours. But the concentration of FABP in the skeletal muscle is 20 times lower than in cardiac tissue (for myoglobin the same content for cardiac and skeletal tissue), that makes FABP to be more cardiac specific than myoglobin. This makes FABP a useful biochemical marker for the early assessment or exclusion of AMI. FABP also appears to be a useful plasma marker for the estimation of myocardial infarct size.

Physical Appearance:

Sterile filtered liquid formulation.

Formulation:

50mM phosphate borate buffer pH-8

Stability:

Recombinant FABP although stable at 10C for 7 days, should be stored desiccated below -20 C for long term periods .
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant FABP.

Applications:

Immunoassays and western blot.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:FABP3

Protein synonyms/aliases:

Fatty acid-binding protein, heart (H-FABP)
    (Heart-type fatty acid-binding protein)
    (Muscle fatty acid-binding protein)
    (M-FABP)
    (Mammary-derived growth inhibitor)
    (MDGI).

Protein Family:

Belongs to the fatty-acid binding protein (FABP)

Protein Domains:

Domains:
•IPR000566   Lipocalin-related protein and Bos/Can/Equ allergen

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Fenofibrate improves insulin sensitivity in connection with intramuscular lipid content, muscle fatty acid-binding protein, and beta-oxidation in skeletal muscle.
J Endocrinol 2002 Aug;174(2):321-9
2. Seasonal dynamics of flight muscle fatty acid binding protein and catabolic enzymes in a migratory shorebird.
Am J Physiol Regul Integr Comp Physiol 2002 May;282(5):R1405-13
3. Increase in skeletal muscle fatty acid binding protein (FABPC) content is directly related to weight loss and to changes in fat oxidation following a very low calorie diet.
Diabetologia 2001 Nov;44(11):2013-7
4. Cloning and sequence of the gene encoding the muscle fatty acid binding protein from the desert locust, Schistocerca gregaria.
Insect Biochem Mol Biol 2001 Apr 27;31(6-7):553-62
5. The muscle fatty acid binding protein of spadefoot toad (Scaphiopus couchii).
Comp Biochem Physiol B Biochem Mol Biol 2000 Mar;125(3):347-57
6. Muscle fatty acid-binding protein.
Biochim Biophys Acta 1999 Nov 23;1441(2-3):94-105

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中華民國95年06月06日更新