Source: E.Coli
Catalog No.
CHM-332
Description : Recombinant Human Exodus-2 is also known as SLC (Secondary Lymphoid-Tissue Chemokine), TCA-4 (T-cell activation-4) , and 6Ckine. Recombinant CCL21 produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12219 Dalton. Recombinant CCL-21 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Solubility: It is recommended to reconstitute the lyophilized Exodus-2 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Exodus-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Exodus-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be, Ser-Asp-Gly-Gly-Ala.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's Recombinant Human Exodus-2 is fully biologically active when compared to standard. The activity is determined by the chemoattract of human PBE (peripheral blood eosinophils) at a concentration between 0.1-10ng/ml.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Exodus-2.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of Exodus-2 as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:CCL21
Synonyms:SCYA21
ORFNames:UNQ784/PRO1600
Small inducible cytokine A21 precursor (CCL21)
(Beta chemokine exodus-2)
(6Ckine)
(Secondary lymphoid-tissue chemokine)
(SLC).
Belongs to the intercrine beta (chemokine CC) family.
Domains:
•IPR001811 Small chemokine, interleukin-8-like
•Recombinant Human Eotaxin(CCL11)
•Recombinant Murine Eotaxin(CCL11)
•Recombinant Human Monocyte Chemotactic Protein-4 (CCL13)
•Recombinant Human HCC-1 (CCL14)
•Recombinant Human Macrophage Inflammatory protein-4 (CCL18)
•Recombinant Human Macrophage Inflammatory protein-3 beta (CCL19)
•Recombinant Human I-309 (CCL1)
•Recombinant Human Monocyte Chemotactic Protein-1/MCAF (CCL2)
•Recombinant Murine Monocyte Chemotactic Protein-1 (CCL2)
•Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2)
•Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3)
•Recombinant Murine Macrophage Inflammatory protein-1 alpha (CCL3)
•Recombinant Human Macrophage Inflammatory protein-1 beta (CCL4)
•Recombinant Murine Macrophage Inflammatory protein-1 beta (CCL4)
•Recombinant Human Rantes (CCL5)
•Recombinant Murine C-10 (CCL6)
•Recombinant Human Monocyte Chemotactic Protein-3 (CCL7)
•Recombinant Murine Monocyte Chemotactic Protein-3 (CCL7)
•Recombinant Human Monocyte Chemotactic Protein-2 (CCL8)
