Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-380

Background :

E-selectin (Endothelial leukocyte adhesion molecule 1/ELAM1/ELAM) belongs to a family of divalent cation-dependent carbohydrate-binding glycoproteins or adhesion molecules. Eselectin is expressed on the surface of endothelial cells and mediates the interaction of leukocytes and platelets with endothelial cells during an inflammatory response.

Description :

Recombinant Human E-Selectin is expressed in E. coli containing amino acids 179-557 fused to an amino terminal hexahistidine tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human E-Selectin is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol).

Stability:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Applications:

Recombinant E-Selectin can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
The biological activity of this product has not yet been tested.

Purity:

Greater than 95% (SDS-PAGE). Single band on Western Blot.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:SELE
Synonyms:ELAM1

Protein synonyms/aliases:

E-selectin precursor (Endothelial leukocyte adhesion molecule 1)
    (ELAM-1)
    (Leukocyte-endothelial cell adhesion molecule 2)
    (LECAM2)
    (CD62E antigen).

Protein Family:

Belongs to the selectin/LECAM family.

Protein Domains:

Domains:
•IPR001304   C-type lectin
•IPR013032   EGF-like region
•IPR006209   EGF-like
•IPR000436   Sushi/SCR/CCP

Related proteins:

•Recombinant Human L-Selectin
•Recombinant Human P-Selectin

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Src-family kinases mediate an outside-in signal necessary for beta2-integrins to achieve full activation and sustain firm adhesion of polymorphonuclear leukocytes tethered on E-selectin.
Biochem J 2006 Jan 24;
2. NAD(P)H oxidase-stimulating activity of serum from type 2 diabetic patients with retinopathy mediates enhanced endothelial expression of E-selectin.
Life Sci 2005 Dec 9;
3. CD43 functions as a ligand for E-Selectin on activated T cells.
J Immunol 2005 Dec 15;175(12):8042-50
4. [An interesting change of E-selectin in cimetidine administration during anticancer drug use]
Gan To Kagaku Ryoho 2005 Oct;32(11):1578-9
5. E-selectin genotypes and risk of type 2 diabetes in women: genetic and environmental contributions to serum soluble E-selectin concentrations.
Obes Res 2005 Oct;13(10):1845-7
6. Intermittent high glucose enhances ICAM-1, VCAM-1 and E-selectin expression in human umbilical vein endothelial cells in culture: the distinct role of protein kinase C and mitochondrial superoxide production.
Atherosclerosis 2005 Dec;183(2):259-67

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中華民國95年06月06日更新