Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-331

Description :

Recombinant Human ENA-78 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton.
Recombinant Human ENA-78 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized ENA-78 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized ENA-78 although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution ENA-78 should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be,
Ala- Ala -Val-Leu-Arg. .

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec旧 Recombinant Human ENA-78 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human peripheral blood neutrophils using a concentration of 5-10 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Human Epithelial neutrophil-activating protein 78

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of ENA-78 as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CXCL5
Synonyms:ENA78, SCYB5

Protein synonyms/aliases:

Small inducible cytokine B5 precursor (CXCL5)
    (Epithelial-derivedneutrophil-activating protein 78)
    (Neutrophil-activating peptide ENA-78)
    (ENA-78(1-78))

Precursor's functional components:

ENA-78(8-78)
ENA-78(9-78) .

Protein Family:

Belongs to the intercrine alpha (chemokine CxC)

Protein Domains:

Domains:
•IPR001811   Small chemokine, interleukin-8-like

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Two polymorphisms in the epithelial cell-derived neutrophil-activating peptide (ENA-78) gene.
Dis Markers 2005;21(2):75-7
2. Cyclooxygenase-2-dependent expression of angiogenic CXC chemokines ENA-78/CXC Ligand (CXCL) 5 and interleukin-8/CXCL8 in human non-small cell lung cancer.
Cancer Res 2004 Mar 1;64(5):1853-60
3. Gelatinase B/MMP-9 and neutrophil collagenase/MMP-8 process the chemokines human GCP-2/CXCL6, ENA-78/CXCL5 and mouse GCP-2/LIX and modulate their physiological activities.
Eur J Biochem 2003 Sep;270(18):3739-49
4. Differential regulation of ENA-78 and GCP-2 gene expression in human corneal keratocytes and epithelial cells.
Invest Ophthalmol Vis Sci 2003 Aug;44(8):3432-7
5. Metalloproteases from Pseudomonas aeruginosa degrade human RANTES, MCP-1, and ENA-78.
J Interferon Cytokine Res 2003 Jun;23(6):307-18
6. Expression of the neutrophil-activating CXC chemokine ENA-78/CXCL5 by human eosinophils.
Clin Exp Allergy 2003 Apr;33(4):531-7

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中華民國95年06月06日更新