Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-309

Description :

Elk-1 is a transcription factor that is activated by mitogen-activated protein kinases (MAPKs) and whose main function is the regulation of growth-related proteins in response to extracellular stimuli. Because of the importance of Elk-1 to the regulation of successful cell growth, accurate monitoring of Elk-1 in cells, tissues and animals is crucial for many biomedical research and drug development projects.

Description :

Recombinant Human ELK-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing 122 amino acids (307-428) and having a molecular mass of 60 kDa. This protein is conjugated with N-terminal His-tag and C-terminal myc tag (EQKLISEEDL). Recombinant Elk-1 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant Human ELK-1 is supplied as lyophilized freeze dry powder without additives.

Biological Activity: :

Recombinant human Elk-1 is phosphorylatable in vitro, using either recombinant active ERK1/2, or with immunoprecipitated ERK1/2 from stimulated cells. This phosphorylation can be monitored by Western blot analysis using BioSource旧 phosphorylation site specific antibody directed to Elk-1 [pS383] in conjunction with chemiluminescence detection methods.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Storage:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles

Purity:

Greater than 95% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Ndrg4 enhances NGF-induced ERK activation uncoupled with Elk-1 activation.
J Cell Biochem 2006 Jan 11;
2. PKCalpha expression regulated by Elk-1 and MZF-1 in human HCC cells.
Biochem Biophys Res Commun 2006 Jan 6;339(1):217-25
3. Smooth muscle-specific genes are differentially sensitive to inhibition by Elk-1.
Mol Cell Biol 2005 Nov;25(22):9874-85
4. A novel protein Depp, which is induced by progesterone in human endometrial stromal cells activates Elk-1 transcription factor.
Mol Hum Reprod 2005 Jul;11(7):471-6
5. Arsenite induces a cell stress-response gene, RTP801, through reactive oxygen species and transcription factors Elk-1 and CCAAT/enhancer-binding protein.
Biochem J 2005 Nov 15;392(Pt 1):93-102
6. A novel protein Depp, which is induced by progesterone in human endometrial stromal cells activates Elk-1 transcription factor.
Mol Hum Reprod 2005 Jul 8;