Certificate of Analysis and Data Sheet

Source:

Catalog No.
ENZ-260

Description :

Human enteropeptidase is a specific protease that cleaves after the sequence Asp-Asp-Aps-Aps-Lys. The light chain of enteropeptidase has full enzymatic activity. No other protease activity was detected. Human enteropeptidase binds specifically to STI-agarose.

Physical Appearance:

Liquid.

Formulation:

50 mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.

Stability:

One year when stored at -20蚓, three weeks at room temperature.
Please avoid freeze-thaw cycles.

Unit definition:

One unit of human enteropeptidase will cleave 2 mg of thioredoxin/human EGF fusion protein with the Asp-Asp-Asp-Asp-Lys sequence at the joining point in 22 hours at 4蚓, in 16 hours at 25蚓 or in 8 hours at 37蚓.

Assay conditions:

50 mM TRIS-HCl or sodium phosphate (pH 8.0) at 25蚓 with or without CaCl2. The enzyme is active at a pH range of 6.0-9.0.

Dilusion buffers:

50 mM Tris-Hcl, pH 8.0, 500 mM NaCl and 50% glycerol.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of enteropeptidase.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. The tetra-aspartate motif in the activation peptide of human cationic trypsinogen is essential for autoactivation control but not for enteropeptidase recognition.
J Biol Chem 2005 Aug 19;280(33):29645-52
2. Study of secondary specificity of enteropeptidase in comparison with trypsin.
Biochemistry (Mosc) 2004 Aug;69(8):909-17
3. Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli.
Protein Expr Purif 2003 Sep;31(1):133-9
4. Expression of enteropeptidase in differentiated enterocytes, goblet cells, and the tumor cells in human duodenum.
Am J Physiol Gastrointest Liver Physiol 2003 Dec;285(6):G1235-41
5. [New substrates for enteropeptidase. I. Biologically active hepta-nonapeptides]
Bioorg Khim 2003 Mar-Apr;29(2):129-34
6. [Inhibition by IFN-(Asp) 4-Lys-HIV chimeric protein of hydrolysis of the low molecular substrate by the enteropeptidase light chain]
Vopr Med Khim 2002 Nov-Dec;48(6):599-602