Certificate of Analysis and Data Sheet

Source:
Baculovirus, SF9 Insect Cells

Catalog No.
PKA-308

Description :

Recombinant Human EIF2-alpha produced in SF9 is a non-glycosylated, polypeptide chain having a molecular mass of 36 kDa. EIF2-a is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant Human EIF2-a is supplied as lyophilized freeze dry powder without additives.

Biological Activity:

Recombinant human eIF2-α is phosphorylatable in vitro, using either immunoprecipitated active PKR or extracts from IFN-γ stimulated HEK 293 cells. This phosphorylation can be monitored by Western blot analysis using phosphorylation site specific antibody directed to eIF2-α [pS51] in conjunction with chemiluminescence detection methods.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Storage:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Reconstitution Recommendation:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human eIF2-α in phosphate buffered saline, to a concentration of 0.2-1.0 mg/mL. Following reconstitution, the protein solution should be supplemented with 1 mM β-mercaptoethanol (final concentration) and 10% glycerol (final concentration) to prevent precipitation.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.