Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-291

Background:

Cathepsin D is synthesized as a 54kDa precursor, which is proteolytically processed to an intermediate 48kDa single chain, which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and xonnective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types. Cathepsin D is expressed in epithelial cells as well as in macrophages.

Description :

Recombinant Cathepsin shows a band at 48kDa on SDS-PAGE commassie blue stained gel. Recombinant Cathepsin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered liquid.

Formulation:

100ug/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability:

Store vial at -20C^o to -80C^o. When stored at the recommended temperature, Cathepsin is stable for 12 months.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates:

Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Cathepsin D.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CTSD

Protein synonyms/aliases:

Cathepsin D precursor (EC 3.4.23.5)

Precursor's functional components:

Cathepsin D lightchain
Cathepsin D heavy chain .

Protein Family:

Belongs to the peptidase A1 family.

Protein Domains:

Domains:
•IPR012848   Propeptide, peptidase A1
•IPR001461   Peptidase A1, pepsin

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Activity of cathepsin D and alpha(1)-antitrypsin in the blood serum of patients with mammary carcinoma.
Exp Oncol 200 5 Sep;27(3):233-7
2. Clinical prognostic factors and expression of cathepsin D in endometrioid adenocarcinoma.
Georgian Med News 2005 Sep;(9):27-31
3. Expression of hypoxia-inducible factor 1alpha and cathepsin D in pituitary adenomas.
Endocr Pathol 2005 Summer;16(2):123-31
4. Effect of cathepsin D and prostate specific antigen on latent transforming growth factor-beta in breast cancer c ell lines.
Malays J Pathol 2003 Dec;25(2):129-34
5. Matrix metalloproteinase-12 and cathepsin D expression in pulmonary macrophages and dendritic cells of cigarette smoke-exposed mice.
Int Arch Allergy Immunol 2005 Oct;138(2):169-79
6. Comparative analysis of hexosaminidase and cathepsin D expression in synovial fluid of patients with rheumatoid arthritis and traumatized joints.
Clin Exp Rheumatol 2005 Sep-Oct;23(5):725-6

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中華民國95年06月06日更新