Certificate of Analysis and Data Sheet

Source:
SF9 insect cells.

Catalog No.
PKA-317

Background :

SRC family kinases are involved in transducing growth factor signals for cellular differentiation and proliferation in a variety of cell types. The activity of all Src family kinases (SFKs) is controlled by phosphorylation at their C-terminal 527-tyrosine residue by C-terminal SRC kinase, CSK. The C-terminal SRC kinase (CSK) family of protein tyrosine kinases contains two members: CSK and CSK homologous kinase (CHK). Both phosphorylate and inactivate SRC family kinases.
C-terminal SRC kinase (CSK) contains a catalytic domain and a regulatory region, consisting of an SH3 and an SH2 domain.

Description :

Recombinant Human C-SRC produced in Sf9 cells is a glycosylated, polypeptide chain having a molecular mass of 60 KD. Recombinant C-SRC is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

C-SRC is supplied at a concentration of 0.29mg/ml in 50mM Tris, pH 7.6, 0.15 MNaCl, 0.27M sucrose, 1mM DTT, 0.1mM EGTA, 0.2mM PMSF, 1mM benzamidine, 5mM NaF, 10mM β-glycerol phosphate, 0.1mM sodium vanadate and 0.03% Brij-35.

Unit definition:

One unit of C-SRC activity is equal to 1 nmol of phosphate transferred to a peptide corresponding to p34cdc2 (6-20) per minute at 30蚓 with a final ATP concentration of 100 然. Recombinant human active C-SRC also phosphorylates STAT, p34cdc2, gastrin, and cortactin.

Biological Activity:

750 units/mg.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Storage:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:CSK

Protein synonyms/aliases:

Tyrosine-protein kinase CSK (EC 2.7.1.112)
    (C-SRC kinase)
    (Protein-tyrosine kinase CYL).

Protein Family:

Belongs to the Tyr protein kinase family. CSK

Protein Domains:

Domains:
•IPR001452   Src homology-3
•IPR000980   SH2 motif
•IPR000719   Protein kinase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. The ubiquitously expressed Csk adaptor protein Cbp is dispensable for embryogenesis and T-cell development and function.
Mol Cell Biol 2005 Dec;25(23):10533-42
2. C-terminal Src kinase (CSK) and CSK-homologous kinase (CHK)--endogenous negative regulators of Src-family protein kinases.
Growth Factors 2005 Sep;23(3):233-44
3. Coupled motions in the SH2 and kinase domains of Csk control Src phosphorylation.
J Mol Biol 2005 Aug 5;351(1):131-43
4. Functional diversity of Csk, Chk, and Src SH2 domains due to a single residue variation.
J Biol Chem 2005 Jul 8;280(27):25780-7
5. CSK negatively regulates nerve growth factor induced neural differentiation and augments AKT kinase activity.
Exp Cell Res 2005 Jul 1;307(1):1-14
6. Association of Csk to VE-cadherin and inhibition of cell proliferation.
EMBO J 2005 May 4;24(9):1686-95

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中華民國95年06月06日更新