Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HSP-003

Description :

Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein(sHSP also known as the HSP20). They act as molecular chaperones and hold them in in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-B is expressed widely in many tissues and organs and occurs in many neurological diseases.

Description :

Recombinant CRYAB produced in E.Coli is a single,non-glycosylated polypeptide chain containing 175amino acids and having a molecular mass of 20,159 Dalton.
Recombinant CRYAB is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

The protein (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 50mM NaCl and 1mM EDTA.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Sequence:

MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSWFDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHRKYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:CRYAB
Synonyms:CRYA2

Protein synonyms/aliases:

Alpha crystallin B chain (Alpha(B)-crystallin)
    (Rosenthal fibercomponent)
    (Heat-shock protein beta-5)
    (HspB5).

Protein Family:

Belongs to the small heat shock protein (HSP20)

Protein Domains:

Domains:
•IPR003090   Crystallin, N-terminal
•IPR002068   Heat shock protein Hsp20

Related proteins:

•Recombinant Human Crystallin Alpha A
•Recombinant Human Heat Shock Protein 27kD
•Recombinant Human Heat Shock Protein 20
•Recombinant Human Heat Shock Protein 22

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Mitochondrial dysfunction and apoptosis underlie the pathogenic process in alpha-B-crystallin desmin-related cardiomyopathy.
Circulation 2005 Nov 29;1 12(22):3451-61
2. Nuclear import of {alpha}B-crystallin is phosphorylation-dependent and hampered by hyperphosphorylation of the myopathy-related mutant R120G.
J Biol Chem 2005 Nov 4;280(44):37139-48
3. Calcium-activated RAF/MEK/ERK signaling pathway mediates p53-dependent apoptosis and is abrogated by alpha B-crystallin through inhibition of RAS activation.
Mol Biol Cell 2005 Sep;16(9):4437-53
4. Alpha B-crystallin, a new independent marker for poor prognosis in head and neck cancer.
Laryngoscope 2005 Jul;115(7):1239-42
5. (alpha)B-crystallin in cerebrospinal fluid of patients with multiple sclerosis.
Clin Chim Acta 2005 Jun;356(1-2):95-101
6. The small heat shock protein alpha B-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3.
J Biol Chem 2005 Mar 25;280(12):11059-66

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中華民國95年06月06日更新