Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-307

Description :

Recombinant Human CREB produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 2-328 and having a molecular mass of 81 KD. This protein is the full-length form and produced as a maltose binding protein (MBP) fusion protein with a poly His-tag. CREB is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human CREB is supplied as lyophilized freeze dry powder without additives.

Biological Activity:

Recombinant human CREB is phosphorylatable in vitro, using either recombinant Protein Kinase A, or Rsk immunoprecipitated from stimulated cells. This phosphorylation can be monitored by ELISA using CREB [pS133] phosphoELISA or by Western blotting using an anti-CREB [pS133] phosphorylation site specific antibody in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Storage:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. A Nitric Oxide Signaling Pathway Controls CREB-Mediated Gene Expression in Neurons.
Mol Cell 2006 Jan 20;21(2):283-294
2. Target Structure-Based Discovery of Small Molecules that Block Human p53 and CREB Binding Protein Association.
Chem Biol 2006 Jan;13(1):81-90
3. Phosphorylation state of CREB in the rat hippocampus: A molecular switch between spatial novelty and spatial familiarity?
Neurobiol Learn Mem 2006 Jan 17;
4. Global transcriptional coactivators CREB-binding protein and p300 are highly essential collectively but not individually in peripheral B-cells.
Blood 2006 Jan 19;
5. Docosahexaenoic Acid Consumption Inhibits Deoxynivalenol-Induced CREB/ATF1 Activation and IL-6 Gene Transcription in Mouse Macrophages.
J Nutr 2006 Feb;136(2):366-72
6. Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in rats.
J Zhejiang Univ Sci B 2006 Feb;7(2):107-13