Certificate of Analysis and Data Sheet

Source:
Pichia Pastoris.

Catalog No.
CKI-271

Background:

The three isoenzymes (MM, MB, and BB) are found in muscle, cardiac and brain tissues. These recombinant proteins are ideal for calibrating diagnostic instruments and researching neuromuscular diseases. Creatine Kinases can be used for indications in many neuromuscular applications. These disorders include cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle's disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases, and other that create elevated or reduced levels of Creatine Kinases.

Description :

Recombinant Human Creatine Kinase MM produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. Recombinant Human CKMMITI is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered colourless liquid formualtion.

Formulation:

Each mg of protein protein contains 20mM Tris pH-8, 1mM EDTA and 1mM DTT.

Stability:

Recombinant Human CKMMITI although stable at 15⁰C for 7 days, should be stored desiccated below -18⁰C.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 痠ole creatine phosphate was 500IU/mg at 37 degrees celsius.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human CKMMITI.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Studies on the creatine kinase MM isoforms of normal and Duchenne muscular dystrophic patients.
Chin Med J (Engl) 1998 Jan;111(1):75-7
2. [Serum creatine kinase MM isoforms and lactate dehydrogenase isoenzymes in patients with non-traumatic acute rhabdomyolysis]
An Med Interna 1998 Jan;15(1):1 3-7
3. Use of serum creatine kinase MM isoforms for predicting the progression of left ventricular dilation in patients with hypertrophic cardiomyopathy.
Jpn Circ J 1997 Apr;61(4):315-22
4. Cardiac markers in the early hours of acute myocardial infarction: clinical performance of creatine kinase, creatine kinase MB isoenzyme (activity and mass concentration), creatine kinase MM and MB subform ratios, myoglobin and cardiac troponin T.
Scand J Clin Lab Invest 1996 Dec;56(8):701-13
5. [Creatine kinase MM and MB isoforms and their potential in the diagnosis of acute myocardial infarction]
Harefuah 1996 Nov 1;131(9):331-4
6. Study of the heterogeneity of creatine kinase-MM isoforms using western blotting.
Clin Biochem 1995 Oct;28(5):519-25