Certificate of Analysis and Data Sheet

Source:
Pichia Pastoris.

Catalog No.
CKI-270

Background :

The three isoenzymes (MM, MB, and BB) are found in muscle, cardiac and brain tissues. These recombinant proteins are ideal for calibrating diagnostic instruments and researching neuromuscular diseases. Creatine Kinases can be used for indications in many neuromuscular applications. These disorders include cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle's disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases, and other that create elevated or reduced levels of Creatine Kinases.

Description :

Recombinant Human Creatine Kinase CKMBITII produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MB Isoenzyme in ELISA. Recombinant Human Creatine Kinase CKMBITII is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered colourless liquid formualtion.

Formulation:

Each mg of protein (15.8mg/ml) contains 20mM Tris-HCl pH-7, 1mM EDTA and 1mM DTT.

Stability:

Recombinant Human CKMBITII although stable at 15⁰C for 7 days, should be stored desiccated below -18⁰C.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 痠ole creatine phosphate was 500IU/mg at 37 degrees celsius.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human CKMBITII.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Frequency and clinical implications of discordant creatine kinase-MB and troponin measurements in acute coronary syndromes.
J Am Coll Cardiol 2006 Jan 17;47(2):312-8
2. Elevated creatine kinase-MB with normal creatine kinase predicts worse outcomes in patients with acute coronary syndromes: results from 4 large clinical trials.
Am Heart J 2006 Jan;151(1):16-24
3. Age-associated increase in creatine kinase-MB-specific activity in human serum confounds its use as an injury marker.
Heart Lung Circ 2005 Jun;14(2):104-6
4. Can creatine kinase MB le vels predict mortality after cardiac surgery?
Nat Clin Pract Cardiovasc Med 2005 Jun;2(6):280-1
5. Comparison of serum cardiac troponin T and creatine kinase MB isoenzyme mass concentrations in asphyxiated term infants during the first 48 h of life.
J Paediatr Child Health 2005 Jul;41(7):331-7
6. Creatine kinas e-MB elevation following stent implantation.
J Am Coll Cardiol 2005 Jun 7;45(11):1908; author reply 1908-9