Certificate of Analysis and Data Sheet

Source:
Pichia Pastoris.

Catalog No.
CKI-268

Background:

The three isoenzymes (MM, MB, and BB) are found in muscle, cardiac and brain tissues. These recombinant proteins are ideal for calibrating diagnostic instruments and researching neuromuscular diseases. Creatine Kinases can be used for indications in many neuromuscular applications. These disorders include cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle's disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases, and other that create elevated or reduced levels of Creatine Kinases.

Description :

Recombinant Human Creatine Kinase BB produced in Pichia Pastoris is a glycosylated polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to BB Isoenzyme in ELISA. Recombinant Human Creatine Kinase BB is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered colourless liquid formualtion.

Formulation:

Each mg of protein protein contains 10mM Tris pH-6.2, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.

Stability:

Recombinant Human CKMBBI although stable at 15⁰C for 7 days, should be stored desiccated below -18⁰C.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 痠ole creatine phosphate was 500IU/mg at 37 degrees celsius.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human CKMBBI.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Effect of macro-creatine kinase and increased creatine kinase BB on the rapid diagnosis of patients with suspected acute myocardial infarction in the emergency department.
Mil Med 2005 Aug;170(8):648-52
2. Increased creatine kinase BB activity and CKB mRNA expression in patients with hematologic disorders: relation to methylation status of the CKB promoter.
Clin Chim Acta 2005 Nov;361(1-2):135-40
3. Creatine kinase BB in brain in schizophrenia.
World J Biol Psychiatry 2003 Oct;4(4):177-83
4. Proteomic identification of oxidatively modified proteins in Alzheimer's disease brain. Part I: creatine kinase BB, glutamine synthase, and ubiquitin carboxy-terminal hydrolase L-1.
Free Radic Biol Med 2002 Aug 15;33(4):562-71
5. Increased protein oxidation and decreased creatine kinase BB expression and activity after spinal cord contusion injury.
J Neurotrauma 2002 Apr;19(4):491-502
6. Somatosensory potentials, CSF creatine kinase BB activity, and awakening after cardiac arrest.
Neurology 2000 Sep 12;55(5):740-1