Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-211

Description :

Recombinant Human Casein Kinase 2 Holoenzyme is a non-glycosylated polypeptide having a molecular mass of 140 kDa. Recombinant Casein Kinase 2 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human CK2-Holoenzyme is supplied in 50% glycerol.

Unit definition:

1U = 1痠ol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.

Storage:

Recombinant CK2-Holoenzyme should be stored at 4蚓 if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20蚓.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

specific activity > 1U/mg.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Primary and secondary interactions between CK2alpha and CK2beta lead to ring-like structures in the crystals of the CK2 holoenzyme.
Mol Cell Biochem 2005 Jun;274(1-2):3-14
2. [Inhibitory effect and its kinetic analysis of baicalein on recombinant human protein kinase CK2 holoenzyme]
Ai Zheng 2004 Aug;23(8):874-8
3. [Inhibitory effect and kinetic analysis of sodium quercetin-7,4'-disulphate on recombinant human protein kinase CK2 holoenzyme]
Yao Xue Xue Bao 2002 Mar;37(3):165-8
4. [Inhibitory effect of AG1109 on recombinant human protein kinase CK2 holoenzyme]
Ai Zheng 2002 Feb;21(2):153-7
5. Inhibitory effect and its kinetic analysis of tyrphostin AG1478 on recombinant human protein kinase CK2 holoenzyme.
Acta Pharmacol Sin 2002 Jun;23(6):556-61
6. Unique activation mechanism of protein kinase CK2. The N-terminal segment is essential for constitutive activity of the catalytic subunit but not of the holoenzyme.
J Biol Chem 2002 Jun 21;277(25):22509-14