Source:
Sf9 insect cells.
Catalog No.
PRO-390
CENP-B is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENP-B antibodies are present in the sera of up to 80% of patients with CREST syndrome.
Description : Recombinant Human CENP-B produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 dalton.
Recombinant CENP-B is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered colourless solution.
Formulation: Recombinant Human CENP-B is supplied at a of 0.9mg/ml in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.
Stability:
Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.
Purity: Greater than 90% (SDS-PAGE). Immunological functions: 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating concentration: 0.15-0.375 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals
Gene:
Name:CENPB
Major centromere autoantigen B (Centromere protein B)
(CENP-B).
Domains:
•IPR006695 CENP-B, N-terminal DNA-binding
•IPR004875 CENP-B protein
