Certificate of Analysis and Data Sheet
Source:
E.Coli
Catalog No.
PKA-325
Description :
Cyclin-dependent kinase 4 combines with the protein cyclin D to form a protein complex that promotes the passage of cells through the G1 checkpoint in the cell growth cycle. This cellular activity is regulated by the protein p16 produced by p16INK4a. Normally, p16 controls cell growth by inhibiting the activity of the CDK4禇yclin D complex and stopping cells at the G1 checkpoint.
Recombinant Human CDK-4 is expressed in E. coli as a full-length protein having a molecular weight of 38kDa fused to an amino terminal hexahistidine tag.
Formulation:
Recombinant Human cyclin-dependent kinase 4 is supplied in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1%(w/v) SDS, 0.1%(w/v) Bromophenol Blue and 2.5% Glycerol.
Storage:
Store at 4蚓 if entire vial will be used within 1-2 weeks.
Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.
Application:
Recombinant CDK-4 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
The biological activity of this product has not yet been tested.
Purity:
Greater than 95% (SDS-PAGE).
Single band on Western Blot.
Usage:
This material is offered by ProSpec-TechnoGene for research,
laboratory or further evaluation purposes.
Gene:
Name:CDK4
Protein synonyms/aliases:
Cell division protein kinase 4 (EC 2.7.1.37)
(Cyclin-dependent kinase4)
(PSK-J3).
Protein Family:
Belongs to the Ser/Thr protein kinase family.
Protein Domains:
Domains:
•IPR000719 Protein kinase
Protein links:
