Source:
E.Coli
Catalog No.
ENZ-270
Physical Appearance: Sterile Filtered blue solution.
Formulation: CAIII Supplied in 1x Laemmli Buffer: 25mM Tris-HCl pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue and 2.5% Glycerol.
Stability:
Human CAIII although stable at 14蚓 for 1 week, should be stored desiccated below -18蚓. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of CAIII.
content: Protein quantitation was carried out by two independent methods:
(1) 1. UV spectroscopy at 280 nm.
(2) Analysis by RP-HPLC, using a standard solution of CAIII as a Reference Standard.
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:CA3
Carbonic anhydrase 3 (EC 4.2.1.1)
(Carbonic anhydrase III)
(Carbonatedehydratase III)
(CA-III).
Belongs to the alpha-carbonic anhydrase family.
Domains:
•IPR001148 Carbonic anhydrase, eukaryotic
