Certificate of Analysis and Data Sheet

Source:
E. Coli

Catalog No.
PRO-411

Description:

Recombinant Human Bcl-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-218.
Recombinant Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered colorless solution.

Formulation:

The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
Resuspend Bcl-2 in 100ul of 10% Acetic acid (Glacial), over night at 4C.

Stability:

Recombinant Bcl-2 although stable at 12⁰C for 1 week, should be stored desiccated below -18⁰C.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Other available Variants:

Recombinant Human Bcl-2 minus BH1 domain
Recombinant Human Bcl-2 minus BH2 domain
Recombinant Human Bcl-2 minus BH3 domain
Recombinant Human Bcl-2 minus BH4 domain, minus (10-30)
Recombinant Human Bcl-2 NWGR
Recombinant Human Bcl-2 N-terminal 8 kDa
Recombinant Human Bcl-2 N-terminal + C-terminal
Recombinant Human GST-Bcl-XL
Recombinant Human Bcl-XL

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Bcl-2.

Applications:

Input marker or positive control (Western Blotting).
Function study (binding assay).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:BCL2

Protein synonyms/aliases:

Apoptosis regulator Bcl-2.

Protein Family:

Belongs to the Bcl-2 family.

Protein Domains:

Domains:
•IPR003093   Apoptosis regulator Bcl-2 protein, BH4
•IPR000712   Apoptosis regulator Bcl-2, BH

Related proteins:

•Recombinant Mouse BCL-2 Associated X Protein (BAX)

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Direct interaction of Bcl-2 proteins with tubulin.
Biochem Biophys Res Commun 2006 Mar 10;341(2):433-9
2. Oxindole alkaloids from Uncaria tomentosa induce apoptosis in proliferating, G0/G1-arrested and bcl-2-expressing acute lymphoblastic leukaemia cells.
Br J Haematol 2006 Mar;132(5):615-22
3. The Apoptotic Effect of Brucine from the Seed of Strychnos nux-vomica on Human Hepatoma Cells is Mediated via Bcl-2 and Ca2+ Involved Mitochondrial Pathway.
Toxicol Sci 2006 Jan 27;
4. Expression of bcl-2 in papillary thyroid cancers and its prognostic value.
Acta Chir Belg 2005 Nov-Dec;105(6):644-8
5. Ethanolic Neem (Azadirachta indica) Leaf Extract Induces Apoptosis in the Hamster Buccal Pouch Carcinogenesis Model by Modulation of Bcl-2, Bim, Caspase 8 and Caspase 3.
Asian Pac J Cancer Prev 2005 Oct-Dec;6(4):515-20
6. Trichostatin A sensitizes TRAIL-resistant myeloma cells by downregulation of the antiapoptotic Bcl-2 proteins.
Cancer Chemother Pharmacol 2006 Jan 25;:1-7

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中華民國95年06月06日更新