Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-337

Background:

An 11 kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.

Description :

Recombinant Human B2M produced in E.Coli is a non-glycosylated polypeptide chain having a molecular mass of 11.76kDa.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

Recombinant Human Myoglobin, at a concentration of 1 mg/ml, phosphate-buffer saline (pH 7.4) and 0.05% NaN3.

Stability:

Recombinant Beta2 Microglobulin ,although stable at 4C for 3 weeks, should be stored desiccated below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

<

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant B2 Microglobulin.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:B2M
ORFNames:CDABP0092, HDCMA22P

Protein synonyms/aliases:

Beta-2-microglobulin precursor

Precursor's functional components:

Beta-2-microglobulin variantpI 5.3 .

Protein Family:

Protein Domains:

Domains:
•IPR003597   Immunoglobulin C1 type
•IPR007110   Immunoglobulin-like

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Cardiac troponin I and beta 2 microglobulin as risk factors for early-onset atherosclerosis in patients on haemodialysis.
Nephrology (Carlton) 2005 Oct;10(5):453-8
2. Structural stability of amyloid fibrils of beta(2)-microglobulin in comparison with its native fold.
Biochim Biophys Acta 2005 Nov 10;1753(1):64-75
3. The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules.
J Immunol 2005 Oct 15;175(8):4866-74
4. Structural studies reveal that the diverse morphology of beta(2)-microglobulin aggregates is a reflection of different molecular architectures.
Biochim Biophys Acta 2005 Nov 10;1753(1):108-20
5. Expression of neutral glycosphingolipids in the brain, lymphoid organs and lungs of mice lacking beta(2)-microglobulin.
Neuroimmunomodulation 2005;12(5):310-3
6. Beta(2)-microglobulin removal by extracorporeal renal replacement therapies.
Biochim Biophys Acta 2005 Nov 10;1753(1):146-53

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中華民國95年06月06日更新