Certificate of Analysis and Data Sheet

Source:
Pichia Pastoris

Catalog No.
PRO-284

Background:

Angiostatin was observed initially as an angiogenesis inhibitor, specifically inhibiting endothelial cell proliferation Recombinant angiostatin with one less kringle domain (kringle 1-3) acts as an effective inhibitor of angiogenesis induced by angiogenin , FGF basic, and VEGF

Description :

Recombinant Human Angiostatin produced in Pichia Pastoris is a single, non-glycosylated polypeptide chain having a molecular mass of 29711 Dalton. rHuAng K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4.
rHuAng K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth.
Human Angiostatin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered liquid formulation.

Formulation:

1mg/ml containing 0.5X PBS.

Stability:

Recombinant Human Angiostatin K1-3 should be stored at 4⁰c. For long term storage it is recommended to add a carrier protein ( % HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

Inhibitory activity (ED50=70 nm) relative to Kringles 1-4 (ED50=135 nm). Kringles 1-3 acts as inhibitor of endothelial cell growth and angiogenesis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Angiostatin.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Angiostatin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:PLG

Protein synonyms/aliases:

Plasminogen precursor (EC 3.4.21.7)

Precursor's functional components:

Plasmin heavy chain A;Activation peptide
Angiostatin
Plasmin heavy chain A, short form;Plasmin light chain B .

Protein Family:

Belongs to the peptidase S1 family. Plasminogen

Protein Domains:

Domains:
•IPR003014   N/apple PAN
•IPR000001   Kringle
•IPR001254   Peptidase S1 and S6, chymotrypsin/Hap

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Angiostatin is directly cytotoxic to tumor cells at low extracellular pH: a mechanism dependent on cell surface-associated ATP synthase.
Cancer Res 2006 Jan 15;66(2):875-82
2. Inhibition of angiogenesis and tumor progression by hydrodynamic cotransfection of angiostatin K1-3, endostatin, and saxatilin genes.
Cancer Gene Ther 2006 Jan 13;
3. Therapeutic potential of angiostatin in diabetic nephropathy.
J Am Soc Nephrol 2006 Feb;17(2):475-86
4. Tumor shedding of laminin binding protein modulates angiostatin production in vitro and interferes with plasmin-derived inhibition of angiogenesis in aortic ring cultures.
Int J Cancer 2005 Dec 27;
5. EXPRESSION OF ANGIOSTATIN, INTEGRIN alphavbeta3, AND VITRONECTIN IN HUMAN LUNGS IN SEPSIS.
Exp Lung Res 2005 Oct;31(8):771-82
6. Influence of angiostatin and thalidomide on lymphangiogenesis.
Lymphology 2005 Sep;38(3):146-55

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中華民國95年06月06日更新