Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-306

Description :

Recombinant Human ATF-2 produced in E.Coli is a non-glycosylated, polypeptide chain containing 78 amino acids and having a molecular mass of 57 KD. ATF-2 is purified by proprietary chromatographic techniques. This protein is the full-length form of the protein with a GST-tag.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human ATF-2 is supplied as lyophilized freeze dry powder without additives.

Unit definition:

Recombinant human ATF2 is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with immunoprecipitated JNK from stimulated cells. This phosphorylation can be monitored by Western blot analysis using phosphorylation site specific antibody directed to ATF2 [pT71] in conjunction with chemiluminescence detection methods.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Storage:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ATF2
Synonyms:CREB2, CREBP1

Protein synonyms/aliases:

Cyclic AMP-dependent transcription factor ATF-2 (Activatingtranscription factor 2)
    (cAMP response element-binding protein CRE-BP1)
    (HB16).

Protein Family:

Belongs to the bZIP family. ATF subfamily.

Protein Domains:

Domains:
•IPR007087   Zinc finger, C2H2-type
•IPR011616   bZIP transcription factor, bZIP_1

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Cyclin D1 activation through ATF-2 in Reg-induced pancreatic beta-cell regeneration.
FEBS Lett 2006 Jan 23;580(2):585-91
2. The bZip proteins CES-2 and ATF-2 alter the timing of transcription for a cell-specific target gene in C. elegans.
Dev Biol 2006 Jan 15;289(2):456-65
3. TGF-beta1 induces cardiac hypertrophic responses via PKC-dependent ATF-2 activation.
J Mol Cell Cardiol 2005 Oct;39(4):627-36
4. Activation of cyclin D1 by estradiol and spermine in MCF-7 breast cancer cells: a mechanism involving the p38 MAP kinase and phosphorylation of ATF-2.
Oncol Res 2005;15(3):113-28
5. Activation of c-Jun and ATF-2 in primate motor cranial nerve nuclei is not associated with apoptosis following axotomy.
Exp Neurol 2005 Jul;194(1):57-65
6. EBV infection induces expression of the transcription factors ATF-2/c-Jun in B lymphocytes but not in B-CLL cells.
Virus Genes 2005 May;30(3):323-30

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中華民國95年06月06日更新