Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ENZ-307

Background :

This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA.

Description :

Recombinant Human ADAT-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-502 and having a total molecular mass of 57.7 kda.
ADAT-1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

Recombinant Human Adenosine Deaminase tRNA-Specific-1 at 0.1mg/ml, 10 mM Tris, pH 8.0,
0.1% Triton X-100, 0.002% NaN3.

Stability:

Recombinant Human ADAT-1although stable at 14⁰C for 1 week, should be stored desiccated
below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Applications:

?ELISA
?MS
?Inhibition Assays
?Western Blotting

Purity:

Greater than 95.0% as determined by the analysis by reducing and non-reducing SDS-PAGE coomassie blue staining.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ADAT1

Protein synonyms/aliases:

ADAT1 protein.

Protein Family:

Protein Domains:

Domains:
•IPR002466   Adenosine deaminase/editase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Genomic clustering of tRNA-specific adenosine deaminase ADAT1 and two tRNA synthetases.
Mamm Genome 2001 May;12(5):387-93
2. Sequence, genomic organization and functional expression of the murine tRNA-specific adenosine deaminase ADAT1.
Gene 2000 Feb 8;243(1-2):59-66

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中華民國95年06月06日更新