Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ENZ-296

Background :

Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.

Description :

Recombinant Human ACY-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-408 and having a total molecular mass of 46.9 kda. Recombinant Human ACY1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human Aminoacylase-1 at a concentration of 0.1mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.

Stability:

Recombinant ACY-1 although stable at 14⁰C for 1 week, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Applications:

?ELISA
?MS
?Inhibition Assays
?Western Blotting

Purity:

Greater than 95.0% as determined by the analysis by reducing and non-reducing SDS-PAGE coomassie blue staining.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ACY1

Protein synonyms/aliases:

Aminoacylase-1 (EC 3.5.1.14)
    (N-acyl-L-amino-acid amidohydrolase)
    (ACY-1).

Protein Family:

Belongs to the peptidase M20B family.

Protein Domains:

Domains:
•IPR011650   Peptidase dimerisation
•IPR002933   Peptidase M20

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Mutations in ACY1, the Gene Encoding Aminoacylase 1, Cause a Novel Inborn Error of Metabolism.
Am J Hum Genet 2006 Jan 18;78(3)
2. The pig aminoacylase 1 (ACY1) and ribosomal protein L29 (RPL29)/heparin/heparan sulfate interacting protein (HIP) genes are located together at 13q21-- > q22, corresponding to human 3p21.1.
Cytogenet Cell Genet 1999;86(1):56-61
3. The human chromosome 3 gene cluster ACY1-CACNA1D-ZNF64-ATP2B2 is evolutionarily conserved in Ateles paniscus chamek (Platyrrhini, Primates).
Cytogenet Cell Genet 1997;77(3-4):314-7
4. A human gene encoding diazepam-binding inhibitor/acy1-CoA-binding protein: transcription and hormonal regulation in the androgen-sensitive human prostatic adenocarcinoma cell line LNCaP.
DNA Cell Biol 1996 Mar;15(3):197-208
5. Structural studies of lipooligosaccharides from Haemophilus ducreyi ITM 5535, ITM 3147, and a fresh clinical isolate, ACY1: evidence for intrastrain heterogeneity with the production of mutually exclusive sialylated or elongated glycoforms.
J Bacteriol 1995 Sep;177(18):5316-21
6. Synthesis and antimicrobial activity of 1-acyl-2-sulfanilylhydrazine and 1-acy1-2-(4-amino-1-naphthylsulfony1)hydrazine.
Chem Pharm Bull (Tokyo) 1962 Mar;10:210-9

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中華民國95年06月06日更新