Source:
E.Coli
Catalog No.
PRO-379
Background: The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Description: Recombinant Human ACTL6A produced in E.Coli is a non-glycosylated, polypeptide chain containing 430 (1-429) amino acids and having a total molecular mass of 49.3 kda. Recombinant ACTL6A contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.
Physical Appearance: Sterile Filtered clear soultion.
Formulation: Recombinant Human Actin Like 6A at a concentration of 0.1mg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol.
Stability: Recombinant ACTL6A although stable at 140C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Applications:
?ELISA
?Inhibition Assays
?Western Blotting
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:ACTL6A
Synonyms:BAF53, BAF53A
Actin-like protein 6A (53 kDa BRG1-associated factor A)
(Actin-relatedprotein Baf53a)
(ArpNbeta).
Belongs to the actin family.
Domains:
•IPR004000 Actin/actin-like
•Recombinant Human Actin Gamma 1
