Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-377

Background :

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Description :

Recombinant Human ACTG-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids (1-375) and having a total molecular mass of 41.8 kda. Recombinant Human ACTG1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human Actin Gamma 1 at a concentration of 0.1mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.

Stability:

Recombinant Human ACTG-1 although stable at 14⁰C for 1 week, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Applications:

?ELISA
?MS
?Inhibition Assays
?Western Blotting

Purity:

Greater than 95.0% as determined by the analysis by reducing and non-reducing SDS-PAGE coomassie blue staining.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ACTG1
Synonyms:ACTG

Protein synonyms/aliases:

Actin, cytoplasmic 2 (Gamma-actin).

Protein Family:

Belongs to the actin family.

Protein Domains:

Domains:
•IPR004000   Actin/actin-like

Related proteins:

•Recombinant Human Actin-Like 6A

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Differential distribution of beta- and gamma-actin in guinea-pig cochlear sensory and supporting cells.
Hear Res 2005 Sep;207(1-2):22-34
2. The gamma-actin encoding gene from the beta-carotene producer Blakeslea trispora.
FEMS Microbiol Lett 2005 Mar 1;244(1):221-8
3. A novel role for non-muscle gamma-actin in skeletal muscle sarcomere assembly.
Exp Cell Res 2004 Jul 1;297(1):82-96
4. Urogenital tract expression of enhanced green fluorescent protein in transgenic mice driven by a smooth muscle gamma-actin promoter.
J Urol 2004 Feb;171(2 Pt 1):944-9
5. A mutation in the gamma actin 1 (ACTG1) gene causes autosomal dominant hearing lo ss (DFNA20/26).
J Med Genet 2003 Dec;40(12):879-84
6. Mutations in the gamma-actin gene (ACTG1) are associated with dominant progressive deafness (DFNA20/26).
Am J Hum Genet 2003 Nov;73(5):1082-91

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中華民國95年06月06日更新