Certificate of Analysis and Data Sheet

Catalog No.
HIV-107

Source
E.coli

Description:

The protein contains the C- terminus of gp46 and most of p21E of HTLV-I. This non-fusion contains immunodominant fragment ,amino acids (165-440).

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein conc:

1 mg/ml containing 10 mM Na-PO4. pH 6.0, 0.1% SDS and 1 mM DTT.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability :

Five years frozen. One month in solution at room temperature.

Sfecificity:

Immunoreactive with all sera of HTLV-I and HTLV-II infected individuals with antibody response to HTLV envelope.

Applications:

Use as an antigen in ELISA and Western Blots.
Excellent reagent for correct detection of HTLV infections, with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ENV

Protein synonyms/aliases:

Env polyprotein precursor

Precursor's functional components:

Surface glycoprotein GP46
Coatprotein GP21 (Transmembrane glycoprotein GP21) .

Protein Family:

Protein Domains:

Domains:
•IPR002050   ENV polyprotein (coat polyprotein)

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. New insights in HTLV-I phylogeny by sequencing and analyzing the entire envelope gene.
AIDS Res Hum Retroviruses 2005 Jan;21(1):28-42
2. Chimeric synthetic peptides containing two immunodominant epitopes from the envelope gp46 and the transmembrane gp21 glycoproteins of HTLV-I virus.
Biochem Biophys Res Commun 2001 Nov 23;289(1):1-6
3. Targeted expression of HTLV-I envelope proteins in muscle by DNA immunization of mice.
Vaccine 2000 Apr 28;18(21):2212-22
4. Expression, purification and biological properties of the carboxyl half part of the HTLV-I surface envelope glycoprotein.
J Chromatogr B Biomed Sci Appl 2000 Jan 14;737(1-2):85-95
5. HTLV-I associated sicca syndrome in Guadeloupe: lack of relation with a peculiar encoding sequence of surface envelope glycoprotein.
Virus Genes 1998;16(2):195-8
6. Identification of exposed epitopes on the envelope glycoproteins of human T-cell lymphotropic virus type I (HTLV-I)
Int J Cancer 1998 Mar 2;75(5):804-13

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中華民國95年06月06日更新