Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-423

Background:

Human Prostate-Specific Gene-1 (HPG-1) product is a recently discovered membrane-anchored protein of approximately 15 kD. It was detected exclusively in the prostate (19 different tissues were tested).
Antisense-oligonicleotide inhibition of HPG-1 expression inhibited the growth of one prostate cancer cell line (LNCaP) significantly (86%). HPG-1 might be a novel diagnostic and/or therapeutic target.

Description:

Recombinant HPG-1 is a 16 kDa recombinant fusion protein consisting of 127 amino acid residues of the human PG-1 and 9 additional amino acid residues of the His-Tag (bold).
MKHHHHHHHM IKKNLKKLGI EETYLNIIKA IYDRPTASIQ KTENLSTKTG TSQEYHLSPL WFYIVLEILA STIRQEKNLK DIHTEKEEVK LSLFADAMIL YLKKPNDSTR KLLELIKKIH SSCRIKINIH FAFCSQ.

Physical Appearance:

Sterile Filtered lyophilized (freeze-dried) powder.

Formulation:

Sterile filtered and lyophilized from 0.5 mg/ml in 0.05 M MES buffer pH6.5.

Specificity:

The amino acid sequence of the recombinant human PG-1 is 100% homologous to the amino acid sequence of the HPG-1.

Purification Method:

Ni-NTA affinity chromatography

Purity:

>95 % (SDS-PAGE analyzed).

Reconstitution:

Add 0.2 ml of dH2O and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.

Stability/Shelf Life:

Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4蚓 for a limited period of time.
The lyophilized protein remains stable until the expiry date when stored at -20蚓.

Applications:

Western blotting, ELISA

References:

?Herness E. A. and Naz R. K. A novel human prostate-specific gene-1 (HPG-1): Molecular cloning, sequencing and its potential involvement in prostate carcinogenesis.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

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