檔案名稱：rp-1/rHIVIp66pol.html

Recombinant Human HIV-I p66 pol Certificate of Analysis and Data Sheet

Source:
Baculovirus

Catalog No.
HIV-127

Description :

Recombinant HIV-1 p66 pol (reverse transcriptase) has a Molecular weight of 66,000 dalton, glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells.

Physical Appearance:

Sterile Filtered clear solution.

Formulation:

Each ml of protein solution (100ug) contains 10mM Tris pH 8, 140mM sodium chloride and 400mM L-Arginine. .

Stability:

Recombinant HIV-Ip66 although stable at 4⁰C for 3 weeks, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological activity:

Immunoreactive with sera from HIV-1 p66pol infected individuals. Western blots: 0.1-1.0 ug/strip.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of HIV-1.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. The N137 and P140 amino acids in the p51 and the P95 amino acid in the p66 subunit of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase are instrumental to maintain catalytic activity and to design new classes of anti-HIV-1 drugs.
FEBS Lett 2005 Apr 25;579(11):2294-300
2. Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity.
Protein Eng Des Sel 2004 Jul;17(7):581-7
3. Proteolytic processing of an HIV-1 pol polyprotein precursor: insights into the mechanism of reverse transcriptase p66/p51 heterodimer formation.
Int J Biochem Cell Biol 2004 Sep;36(9):1836-47
4. Identification of a putative binding site for [2',5'-bis-O-(tert-butyldimethylsilyl)-beta-D-ribofuranosyl]-3'-spiro-5''-(4''-amino-1'',2''-oxathiole-2'',2''-dioxide)thymine (TSAO) derivatives at the p51-p66 interface of HIV-1 reverse transcriptase.
J Med Chem 2001 Jun 7;44(12):1853-65
5. Factors affecting the dimerization of the p66 form of HIV-1 reverse transcriptase.
Eur J Biochem 2001 Mar;268(5):1163-72
6. Temperature-dependent equilibrium between the open and closed conformation of the p66 subunit of HIV-1 reverse transcriptase revealed by site-directed spin labelling.
J Mol Biol 2000 Aug 25;301(4):1029-39

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中華民國95年06月06日更新