Certificate of Analysis and Data Sheet

Catalog No.
HIV-124

Source
E.coli

Description :

E.coli derived recombinant. The protein contains the full-length sequence of HIV-I immunodominant regions nef 3-190 a.a.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein conc:

1mg/ml, 0.01M Na2CO3, 0.01M Na3EDTA and 0.014M β-ME.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Specificity:

Immunoreactive with all sera of HIV-I infected individuals.

Application:

Antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:nef

Protein synonyms/aliases:

Nef protein (Negative factor)
    (F-protein)
    (3'ORF)

Precursor's functional components:

C-terminal core protein .

Protein Family:

Belongs to the lentiviruses Nef protein family.

Protein Domains:

Domains:
•IPR001558   HIV negative factor Nef

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Regulation of interleukin-18 by THP-1 monocytoid cells stimulated with HIV-1 and Nef viral protein.
Eur Cytokine Netw 2005 Sep;16(3):186-90
2. Evasion of cytotoxic T lymphocytes is a functional constraint maintaining HIV-1 Nef expression.
Eur J Immunol 2005 Nov;35(11):3221-8
3. CD8+ T cell epitope-flanking mutations disrupt proteasomal processing of HIV-1 Nef.
J Immunol 2005 Oct 1;175(7):4618-26
4. The Nef protein of HIV-1 induces loss of cell surface costimulatory molecules CD80 and CD86 in APCs.
J Immunol 2005 Oct 1;175(7):4566-74
5. Library versus library recognition and inhibition of the HIV-1 Nef allelome.
J Am Chem Soc 2005 Sep 7;127(35):12178-9
6. Cytolytic T lymphocytes (CTLs) from HIV-1 subtype C-infected Indian patients recognize CTL epitopes from a conserved immunodominant region of HIV-1 Gag and Nef.
J Infect Dis 2005 Sep 1;192(5):749-59

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中華民國95年06月06日更新