Certificate of Analysis and Data Sheet

Catalog No.
HIV-113

Source
E.coli

Description :

The protein contains the full-length sequence of HIV-I glycoprotein gp41L.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein conc:

1mg/ml, 50mM Tris-HCl, 10mM glutation, 60mM NaCl, 0.25% sarcosyl, 50% glycerol.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20C.

formulation:

1mg/ml, 50mM Tris-HCl pH-6, 60mM NaCl, 10mM glutation, 50% glycerol and 0.25% sarcosyl.

Stability:

Five years frozen. One month in solution at room temperature.

Specificity:

Immunoreactive with all sera of HIV-I infected individuals.

Application:

Antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Label:

Also available:

* Recombinant HIV-Ip24, biotin conjugate.
-Price: $125/100ug, $475/500ug, $900/1000ug.

* Recombinant HIV-Ip24, Horseradish peroxidase labled.
-Price: $125/100ug, $475/500ug, $900/1000ug.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Characterization and HIV-1 fusion inhibitory properties of monoclonal Fabs obtained from a human non-immune phage library selected against diverse epitopes of the ectodomain of HIV-1 gp41.
J Mol Biol 2005 Nov 11;353(5):945-51
2. Conformational changes in HIV-1 gp41 in the course of HIV-1 envelope glycoprotein-mediated fusion and inactivation.
Biochemistry 2005 Sep 20;44(37):12471-9
3. Structure and orientation study of fusion peptide FP23 of gp41 from HIV-1 alone or inserted into various lipid membrane models (mono-, bi- and multibi-layers) by FT-IR spectroscopies and Brewster angle microscopy.
Biochim Biophys Acta 2005 Sep 15;1715(2):81-95
4. The recombinant immunogen with high-density epitopes of ELDKWA and ELDEWA induced antibodies recognizing both epitopes on HIV-1 gp41.
Microbiol Immunol 2005;49(8):703-9
5. The complex antigenicity of a small external region of the C-terminal tail of the HIV-1 gp41 envelope protein: a lesson in epitope analysis.
Rev Med Virol 2005 Nov-Dec;15(6):365-81
6. NK cytotoxicity against CD4+ T cells during HIV-1 infection: a gp41 peptide induces the expression of an NKp44 ligand.
Proc Natl Acad Sci U S A 2005 Aug 2;102(31):10981-6