Certificate of Analysis and Data Sheet

Catalog No.
HIV-116

Source
E.coli

Description :

The protein contains the a 32kD fragment of HIV-I glycoprotein gp41, amino acids:(466-753).

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein conc:

1mg/ml, 20mM PBS pH7.8, 20mM NaCl 0.5M, 1mM DTT and 8M urea.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Five years frozen. One month in solution at room temperature.

Specificity:

Immunoreactive with all sera of HIV-I infected individuals.

Application:

Antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ENV

Protein synonyms/aliases:

Envelope polyprotein GP160 precursor

Precursor's functional components:

Exterior membraneglycoprotein (GP120)
Transmembrane glycoprotein (GP41) .

Protein Family:

Protein Domains:

Domains:
•IPR000777   Envelope glycoprotein GP120
•IPR000328   Envelope Polyprotein GP41

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Characterization and HIV-1 fusion inhibitory properties of monoclonal Fabs obtained from a human non-immune phage library selected against diverse epitopes of the ectodomain of HIV-1 gp41.
J Mol Biol 2005 Nov 11;353(5):945-51
2. Conformational changes in HIV-1 gp41 in the course of HIV-1 envelope glycoprotein-mediated fusion and inactivation.
Biochemistry 2005 Sep 20;44(37):12471-9
3. Structure and orientation study of fusion peptide FP23 of gp41 from HIV-1 alone or inserted into various lipid membrane models (mono-, bi- and multibi-layers) by FT-IR spectroscopies and Brewster angle microscopy.
Biochim Biophys Acta 2005 Sep 15;1715(2):81-95
4. The recombinant immunogen with high-density epitopes of ELDKWA and ELDEWA induced antibodies recognizing both epitopes on HIV-1 gp41.
Microbiol Immunol 2005;49(8):703-9
5. The complex antigenicity of a small external region of the C-terminal tail of the HIV-1 gp41 envelope protein: a lesson in epitope analysis.
Rev Med Virol 2005 Nov-Dec;15(6):365-81
6. NK cytotoxicity against CD4+ T cells during HIV-1 infection: a gp41 peptide induces the expression of an NKp44 ligand.
Proc Natl Acad Sci U S A 2005 Aug 2;102(31):10981-6

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中華民國95年06月06日更新