Certificate of Analysis and Data Sheet

Catalog No.
HIV-122

Source
E.coli

Description :

E.coli derived recombinant.
The protein contains the full-length sequence of HIV-I immunodominant regions from pol protein (Intergrase).

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein conc:

1mg/0.22ml, 50mM Tris-HCL, pH-8, 60mM NaCl, 10mM Glutathione, 0.25 sarkosil and 50% glycerol.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Specificity:

Immunoreactive with all sera of HIV-I infected individuals.

Application:

Antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Label:

Also available:

* Recombinant HIV-Ip24, biotin conjugate.
-Price: $125/100ug, $475/500ug, $900/1000ug.

* Recombinant HIV-Ip24, Horseradish peroxidase labled.
-Price: $125/100ug, $475/500ug, $900/1000ug.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:GAG
andName:POL

Protein synonyms/aliases:

Gag-Pol polyprotein (Pr160Gag-Pol)

Precursor's functional components:

Matrix protein p17 (MA);Capsid protein p24 (CA)
p2 spacer peptide
Nucleocapsid protein*(NC*)
Transframe peptide (TF)
    (p6 pol)
Protease (EC 3.4.23.16)
    (Retropepsin)
    (PR)
Reverse transcriptase/ribonuclease H (EC 2.7.7.49)
    (EC 2.7.7.7)
    (EC 3.1.26.4)
    (p66 RT)
p51 RT
p15
Integrase (IN) .

Protein Family:

Protein Domains:

Domains:
•IPR001995   Peptidase A2A, retrovirus, catalytic
•IPR000071   Immunodeficiency lentiviral matrix
•IPR000477   RNA-directed DNA polymerase (Reverse transcriptase)
•IPR000721   Retroviral nucleocapsid protein Gag
•IPR001878   Zinc finger, CCHC-type
•IPR001878   Zinc finger, CCHC-type
•IPR010661   Reverse transcriptase thumb
•IPR001995   Peptidase A2A, retrovirus, catalytic
•IPR010659   Reverse transcriptase connection
•IPR002156   Ribonuclease H
•IPR003308   Integrase, N-terminal zinc-binding
•IPR000477   RNA-directed DNA polymerase (Reverse transcriptase)
•IPR010661   Reverse transcriptase thumb
•IPR001584   Integrase, catalytic region
•IPR001037   Retroviral integrase, C-terminal
•IPR010659   Reverse transcriptase connection
•IPR002156   Ribonuclease H
•IPR003308   Integrase, N-terminal zinc-binding
•IPR001584   Integrase, catalytic region
•IPR001037   Retroviral integrase, C-terminal

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Seeing is believing: structure of the catalytic domain of HIV-1 integrase in complex with human LEDGF/p75.
Proc Natl Acad Sci U S A 2005 Dec 6;102(49):17543-4
2. Constructing HIV-1 integrase tetramer and exploring influences of metal ions on forming integrase-DNA complex.
Biochem Biophys Res Commun 2005 Nov 11;337(1):313-9
3. Targeting HIV-1 integrase with aptamers selected against the purified RNase H domain of HIV-1 RT.
Biochimie 2005 Sep-Oct;87(9-10):911-9
4. A series of 5-(5,6)-dihydrouracil substituted 8-hydroxy-[1,6]naphthyridine-7-carboxylic acid 4-fluorobenzylamide inhibitors of HIV-1 integrase and viral replication in cells.
Bioorg Med Chem Lett 2005 Oct 15;15(20):4550-4
5. Model of full-length HIV-1 integrase complexed with viral DNA as template for anti-HIV drug design.
J Comput Aided Mol Des 2004 Dec;18(12):739-60
6. Synthesis and biological evaluation of geminal disulfones as HIV-1 integrase inhibitors.
J Med Chem 2005 Jul 14;48(14):4526-34

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中華民國95年06月06日更新