Certificate of Analysis and Data Sheet

Catalog No.
HIV-139

Source
E.coli

Description :

The protein contains the a fragment of HIV-II coat protein gp36 ,amino acids:(390-702). MW=34 kD.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein conc:

1mg/ml, 20mM PBS pH 7.8, NaCl 0.5M, DTT 1mM, 8M urea and 0.4M imidasole.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Five years frozen. One month in solution at room temperature.

Specificity:

Immunoreactive with all sera of HIV-I infected individuals.

Application:

Antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. HIV-2 transmembrane protein gp36 binds to the putative cellular receptor proteins P45 and P62.
Immunobiology 2000 Jan;201(3-4):317-22
2. HIV-2 transmembrane protein gp36 like HIV-1 gp41 binds to human lymphocytes and monocytes.
AIDS 1995 Oct;9(10):1193-4
3. Insertion of a short human immunodeficiency virus (HIV)-2 gp36 sequence into an HIV-1 p24 recombinant protein results in a polypeptide with potent and TCRBV-restricted T cell triggering activity.
Eur J Immunol 1995 Jan;25(1):218-25