Certificate of Analysis and Data Sheet

Catalog No.
HIV-101

Source E.coli

Description:

This antigen is an E.coli-derived recombinant protein that composes all of the reported immunogenic determinants found in gp41 and a the C-terminus of gp120. The gene encoding this fusion protein was synthesized using codons optimized for E.coli expression and doesn急 represent a linear HIV-I envelope sequence. rHIV-I is a non-glycosylated, 233 aa polypeptide chain, having a molecular mass of 27275.88, pI=9.68.

Physical Appearance :

Liquid.

Formulation & packaging:

The antigen is dissolved in 0.5X PBS and contains 0.05% SDS.

Purity:

Greater than 98.0% as determined by HPLC analysis:

Stability:

Reconstituted rHIV-I (in solution) is best stored refrigerated at 4蚓 under strile conditions and used within a month.

Biological Activity:

This antigen was thoroughly tested in various tests as well as ELISA using the standard BBI serum panels.
All antibody detection tests on this antigen were as sensitive or more than the standard Abott third generation tests.

Protein Determination:

Analysis by RP-HPLC, using a calibrated solution of rHIV-I as a Reference Standard.

Applications:

Antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:ENV

Protein synonyms/aliases:

Envelope polyprotein GP160 precursor

Precursor's functional components:

Exterior membraneglycoprotein (GP120)
Transmembrane glycoprotein (GP41) .

Protein Family:

Protein Domains:

Domains:
•IPR000777 Envelope glycoprotein GP120
•IPR000328 Envelope Polyprotein GP41

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Structure of a V3-containing HIV-1 gp120 core.
Science 2005 Nov 11;310(5750):1025-8
2. The anti-HIV activity of ADS-J1 targets the HIV-1 gp120.
Virology 2005 Dec 5;343(1):141-9
3. Differential involvement of p38 and JNK MAP kinases in HIV-1 Tat and gp120-induced apoptosis and neurite degeneration in striatal neurons.
Neuroscience 2005;135(3):781-90
4. Characterization of antibody responses to purified HIV-1 gp120 glycoproteins fused with the molecular adjuvant C3d.
Virology 2005 Sep 30;340(2):277-84
5. Morphine exacerbates HIV-1 viral protein gp120 induced modulation of chemokine gene expression in U373 astrocytoma cells.
Curr HIV Res 2005 Jul;3(3):277-88
6. Identification of N-phenyl-N'-(2,2,6,6-tetramethyl-piper idin-4-yl)-oxalamides as a new class of HIV-1 entry inhibitors that prevent gp120 binding to CD4.
Virology 2005 Sep 1;339(2):213-25

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中華民國95年06月06日更新