Certificate of Analysis and Data Sheet
Source:
E.Coli
Catalog No.
CYT-202
Background:
Description:
Recombinant HGH produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22260.01 Dalton.
Recombinant HGH is purified by proprietary chromatographic techniques.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
TEach mg HGH contains 5mg mannitol, 1mg glycine and 0.226mg sodium phosphate dibasic.
Solubility:
It is recommended to reconstitute the lyophilized Growth Hormone in sterile 18MΩ-cm H
2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Recombinant GH although stable at room temperature for 3 weeks, should be stored desiccated below -18
0C. Upon reconstitution HGH should be stored at 4
0C between 2-7 days and for future use below -18
0C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ile.
Dimers and aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity:
ProSpec旧 Recombinant Human Growth Hormone is fully biologically active when compared to World Health Organization (WHO) reference standard which is 3 units/mg.
Endotoxin:
Less than 0.1 ng/ug (IEU/ug) of Recombinant Human GH.
Protein content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.72 as the extinction
coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE
computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:GH1
Protein synonyms/aliases:
Somatotropin precursor (Growth hormone)
(GH)
(GH-N)
(Pituitary growthhormone)
(Growth hormone 1).
Protein Family:
Belongs to the somatotropin/prolactin family.
Protein Domains:
Domains:
•IPR001400 Somatotropin hormone
Related proteins:
•Recombinant Human Prolactin
•Recombinant Ovine Prolactin
•Recombinant Human Placental-Corr Growth Hormone-22K
•Recombinant Human Pituitary Growth Hormone-20K
•Recombinant Rat Growth Hormone
•Recombinant Ovine Growth Hormone
•Recombinant bream Growth Hormone
Protein links: