Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HEV-235

Background:

Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function.

Description :

The protein is a mosiac of 12 immunodominant HEV ORF2-ORF3 fragments. Hepatitis E Virus proteins are purified by proprietary chromatographic techniques.

Purification method:

Purified on Sepharose by proprietary chromatographic technique.

Purity:

Recombinant HEV is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml in 25mM Tris-HCl, 1mM EDTA, 0.5M urea, 50% glycerol.

Storage:

Recombinant HEV is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HEV is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HEV-infected individuals

Application:

Recombinant HEV Antigen may be used in ELISA and Western blots, excellent for detection of HEV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. The 41-amino-acid C-terminal region of the hepatitis E virus ORF3 protein interacts with bikunin, a kunitz-type serine protease inhibitor.
J Virol 2005 Sep;79(18):12081-7
2. The ORF3 protein of hepatitis E virus interacts with liver-specific alpha1-microglobulin and its precursor alpha1-microglobulin/bikunin precursor (AMBP) and expedites their export from the hepatocyte.
J Biol Chem 2004 Jul 9;279(28):29308-19
3. [Purification and immunological characterization of hepatitis E vir us recombinant chimeric antigen encoded by ORF2 fragments and ORF3]
Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2001 Aug;23(4):382-5
4. Self-association and mapping of the interaction domain of hepatitis E virus ORF3 protein.
J Virol 2001 Mar;75(5):2493-8
5. The ORF3 protein of hepatitis E virus is a phosphoprotein that associates with the cytoskeleton.
J Virol 1997 Dec;71(12):9045-53
6. Expression and characterization of the hepatitis E virus ORF3 protein in the methylotrophic yeast, Pichia pastoris.
Gene 1997 Apr 29;190(1):63-7