Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HEV-273

Background:

Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function.

Description :

The protein contains immunodominant HEV ORF2 fragment, amino acids:(92-123). Hepatitis E Virus proteins are purified by proprietary chromatographic techniques.

Purification method:

Purified on Sepharose by proprietary chromatographic technique.

Purity:

Recombinant HEV is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml in 25mM Tris-HCl, 1mM EDTA, 0.5M urea, 50% glycerol.

Storage:

Recombinant HEV is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HEV is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HEV-infected individuals

Application:

Recombinant HEV Antigen may be used in ELISA and Western blots, excellent for detection of HEV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

ORFNames:ORF2

Protein synonyms/aliases:

Structural protein 2 precursor.

Protein Family:

Protein Domains:

Domains:
•IPR004261   Hepatitis E virus structural protein 2

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. A truncated ORF2 protein contains the most immunogenic site on ORF2: antibody responses to non-vaccine sequences following challenge of vaccinated and non-vaccinated macaques with hepatitis E virus.
Vaccine 2005 May 2;23(24):3157-65
2. The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA.
J Virol 2004 Jan;78(1):320-8
3. [Purification and immunological characterization of hepatitis E virus recombinant chimeric antigen encoded by ORF2 fragments and ORF3]
Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2001 Aug;23(4):382-5
4. [Preliminary evidence that a hepatitis E virus (HEV) ORF2 recombinant protein protects cynomolgus macaques against challenge with wild-type HEV]
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2002 Mar;16(1):31-2
5. The full-length and N-terminal deletion of ORF2 protein of hepatitis E virus can dimerize.
Biochem Biophys Res Commun 2001 Aug 10;286(1):214-21
6. Augmentation of immune responses to hepatitis E virus ORF2 DNA vaccination by codelivery of cytokine genes.
Viral Immunol 2000;13(2):169-78

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新