Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HDV-234

Background:

Hepatitis delta virus (HDV) contains a circular RNA which encodes a single protein, hepatitis delta antigen (HDAg). HDAg exists in two forms, a small form (S-HDAg) and a large form (L-HDAg). S-HDAg can transactivate HDV RNA replication. Post-translational modifications, such as phosphorylation and acetylation, of S-HDAg can modulate HDV RNA replication. The RNAs undergo different forms of posttranscriptional RNA processing. Transcripts of both the genomic RNA and its exact complement, the antigenomic RNA, undergo ribozyme cleavage and RNA ligation. In addition, antigenomic RNA transcripts can undergo 5' capping, 3' polyadenylation, and even RNA editing by an adenosine deaminase

Description :

The protein contains the HDV immunodominant regions,amino acids:(1-108)(151-209). Hepatitis D Virus proteins are purified by proprietary chromatographic techniques.

Purification method:

Purified from inclusion bodies by proprietary chromatographic technique.

Purity:

Recombinant HDV is >90% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml containing 10 mM CBB, pH 10.0, NaCl 100mM, 50% glycerol.

Storage:

Recombinant HDV is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HDV is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HDV-infected individuals

Application:

Recombinant HDV Antigen may be used in ELISA and Western blots, excellent for detection of HDV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Protein synonyms/aliases:

Large delta antigen (p27)
    (HDAg-L)

Precursor's functional components:

Small delta antigen(p24)
    (HDAg-S) .

Protein Family:

Belongs to the hepatitis delta antigen family.

Protein Domains:

Domains:
•IPR002506   Hepatitis delta virus delta antigen

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. General acid catalysis by the hepatitis delta virus ribozyme.
Nat Chem Biol 2005 Jun;1(1):45-52
2. Chronic hepatitis delta virus infection in Van region of eastern Turkey.
Turk J Gastroenterol 2005 Mar;16(1):17-20
3. Evolution of hepatitis delta virus RNA genome following long-term replication in cell culture.
J Virol 2005 Nov;79(21):13310-6
4. Clevudine inhibits hepatitis delta virus viremia: a pilot study of chronically infected woodchucks.
Antimicrob Agents Chemother 2005 Oct;49(10):4396-9
5. Epidemiology and clinical pattern of hepatitis delta virus infection in Pakistan.
J Gastroenterol Hepatol 2005 Oct;20(10):1503-7
6. [Diagnostic tests: Hepatitis delta virus (HDV) marker]
Nippon Rinsho 2005 Jul;63 Suppl 7:418-20

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中華民國95年06月06日更新