Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HCV-242

Background:

The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood.Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways
In addition,HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.

Description :

The protein contains the HCV core nucleocapsid immunodominant regions. Hepatitis C Virus core proteins are purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Recombinant HCV core 3 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml in 25mM Tris-Hcl pH 8, 1mM EDTA, 1.5M urea and 50% glycerol.

Storage:

Recombinant HCV Core is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HCV Core is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HCV-infected individuals

Application:

Recombinant HCV Core Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Expression of hepatitis C virus core protein in hepatocytes does not modulate proliferation or apoptosis of CD8+ T cells.
Yonsei Med J 2005 Dec 31;46(6):827-34
2. Is an assay for simultaneous detection of hepatitis C virus core antigen and antibody a valuable alternative to nucleic acid testing?
Transfusion 2005 Dec;45(12):1965-72
3. Hepatitis C virus core protein inhibits tumor suppressor protein promyelocytic leukemia function in human hepatoma cells.
Cancer Res 2005 Dec 1;65(23):10830-7
4. SOCS1 and SOCS3 are targeted by hepatitis C virus core/gC1qR ligation to inhibit T-cell function.
J Virol 2005 Dec;79(24):15417-29
5. Activation of Refractory T Cell Responses against Hepatitis C Virus Core Protein by Ablation of Interfering Hydrophobic Domains.
Mol Ther 2006 Feb;13(2):338-46
6. Analysis of hepatitis C virus core antigenemia in Saudi drug users.
Saudi Med J 2005 Oct;26(10):1645-6