檔案名稱：rp-1/rHCVNS5G2.html

Recombinant Hepatitis C Virus NS5enotype??/h1> Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
HCV-229

Background:

The hepatitis C virus (HCV) NS5A gene product is a phosphorylated 56- to 58-kD nonstructural protein that displays a multitude of activities related to enhancement of viral pathogenesis. Although associated with other viral encoded proteins as part of the viral replicase complex positioned on the cytoplasmic side of the endoplasmic reticulum, a role for NS5A in viral replication has not been defined.Truncated versions of NS5A can act as transcriptional activators, while other recently characterized interactions of NS5A with cellular proteins indicate its pleiotropic role in HCV-host interactions.
It has been shown that NS5B is a membrane-associated protein, which contains a C-terminal domain comprising 21 hydrophobic amino acids that is responsible for membrane anchorage. NS5B may form a complex with cellular proteins or other HCV nonstructural proteins, including NS3, the viral protease and helicase; NS4A, a cofactor of NS3 protease activity; and NS5A, a phosphoprotein containing a putative interferon sensitivity region. Although the HCV replication mechanism is not clearly understood, the essential role of NS5B polymerase in the HCV replication and infection process has been demonstrated in chimpanzees. Accordingly, it has been viewed as an attractive target for antiviral intervention.

Description :

The protein contains the HCV NS5 Genotype 1 immunodominant regions. Hepatitis C Virus NS5 proteins are purified by proprietary chromatographic techniques.

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Recombinant HCV NS5 is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Formulation:

1mg/ml, in 50 mM Tris. pH 8.0 and 5 mM EDTA

Storage:

Recombinant HCV NS5 is shipped at ambient temperature. Upon arrival, Store at -20C.

Stability:

Recombinant HCV NS5 is stable 5 years frozen, One month in solution at room temperature.

Specificity:

Immunoreactive with sera of HCV-infected individuals

Application:

Recombinant HCV NS5 Antigen may be used in ELISA and Western blots, excellent for detection of HCV with minimal specificity problems.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Function follows form: the structure of the N-terminal domain of HCV NS5A.
Hepatology 2005 Sep;42(3):732-5
2. Inhibitors of HCV NS5B polymerase. Part 2: Evaluation of the northern region of (2Z)-2-benzoylamino-3-(4-phenoxy-phenyl)-acrylic acid.
Bioorg Med Chem Lett 2005 Jun 2;15(11):2812-8
3. Inhibitors of HCV NS5B polymerase. Part 1: Evaluation of the southern region of (2Z)-2-(benzoylamino)-3-(5-phenyl-2-furyl)acrylic acid.
Bioorg Med Chem Lett 2005 May 16;15(10):2481-6
4. Hepatitis C virus (HCV) NS5A protein downregulates HCV IRES-dependent translation.
J Gen Virol 2005 Apr;86(Pt 4):1015-25
5. Inhibitors of HCV NS5B polymerase: synthesis and structure-activity relationships of N-1-heteroalkyl-4-hydroxyquinolon-3-yl-benzothiadiazines.
Bioorg Med Chem Lett 2005 Mar 15;15(6):1577-82
6. Characterization of a HCV NS5A protein derived from a patient with hepatoma.
Biochem Biophys Res Commun 2005 Feb 11;327(2):516-22

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中華民國95年06月06日更新